DIO1 Antibody – Rabbit Polyclonal

Description: Rabbit IgG polyclonal antibody for DIO1 detection. Tested with WB, FCM, Direct ELISA in Human; Mouse; Rat.

Background: Type I iodothyronine deiodinase is a protein that in humans is encoded by the DIO1 gene. It is mapped to 1p32.3. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

1. Berry, M. J., Banu, L., Larsen, P. R. Type I iodothyronine deiodinase is a selenocysteine-containing enzyme. Nature 349: 438-440, 1991.
2. Berry, M. J., Grieco, D., Taylor, B. A., Maia, A. L., Kieffer, J. D., Beamer, W., Glover, E., Poland, A., Larsen, P. R. Physiological and genetic analyses of inbred mouse strains with a type I iodothyronine 5-prime deiodinase deficiency. J. Clin. Invest. 92: 1517-1528, 1993.
3. de Jong, F. J., Peeters, R. P., den Heijer, T., van der Deure, W. M., Hofman, A., Uitterlinden, A. G., Visser, T. J., Breteler, M. M. B. The association of polymorphisms in the type 1 and 2 deiodinase genes with circulating thyroid hormone parameters and atrophy of the medial temporal lobe. J. Clin. Endocr. Metab. 92: 636-640, 2007.

Small volumes of anti-DIO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1753109 is an antibody targeting DIO1. Reactive in Human, Mouse, Rat. Host: Rabbit.