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ZNFN1A2/HELIOS/IKZF2 (IKZF2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753120

Description: Rabbit IgG polyclonal antibody for ZNFN1A2/HELIOS/IKZF2 detection. Tested with WB, FCM, Direct ELISA in Human; Mouse; Rat.Background: Zinc finger protein Helios is a protein that in humans is encoded by the IKZF2 gene. This gene is mapped to… More details

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Product Overview

Online Datasheet in PDF
Product Name
ZNFN1A2/HELIOS/IKZF2, Polyclonal Antibody
Full Product Name
Anti-ZNFN1A2/HELIOS/IKZF2 Antibody
Product Gene Name
Product Synonym Names
Zinc finger protein Helios; Ikaros family zinc finger protein 2; IKZF2; HELIOS, ZNFN1A2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
606234
3D Structure
ModBase 3D Structure for Q9UKS7
Immunogen
E Coli-derived human ZNFN1A2/HELIOS/IKZF2 recombinant protein (Position: M1-R477).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IKZF2 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-IKZF2 antibody
WB: 0.1-0.25ug/ml (Human, Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-IKZF2 antibody

Rabbit Anti-IKZF2 Polyclonal Antibody – Cat# MBS1753120

Western Blot (WB) of anti-IKZF2 antibody

Figure 1. Western blot analysis of IKZF2 using anti-IKZF2 antibody.
Rabbit Anti-IKZF2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS1753120

Product References and Citations for anti-IKZF2 antibody

1. Chessum, L., Matern, M. S., Kelly, M. C., Johnson, S. L., Ogawa, Y., Milon, B., McMurray, M., Driver, E. C., Parker, A., Song, Y., Codner, G., Esapa, C. T., and 11 others. Helios is a key transcriptional regulator of outer hair cell maturation. Nature 563: 696-700, 2018.
2. Gross, M. B. Personal Communication. Baltimore, Md. 2/15/2012.
3. Hosokawa, Y., Maeda, Y., Seto, M. Human Helios, an Ikaros-related zinc finger DNA binding protein: cDNA cloning and tissue expression pattern. Immunogenetics 50: 106-108, 1999.

Other Notes

Small volumes of anti-IKZF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IKZF2 purchase

MBS1753120 is an antibody targeting IKZF2. Also known as Anti-ZNFN1A2/HELIOS/IKZF2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IKZF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
zinc finger protein Helios isoform 2
NCBI Official Synonym Full Names
IKAROS family zinc finger 2
NCBI Official Symbol
IKZF2
NCBI Official Synonym Symbols
ANF1A2; HELIOS; ZNF1A2; ZNFN1A2
UniProt Protein Name
Zinc finger protein Helios
UniProt Synonym Protein Names
Ikaros family zinc finger protein 2
Protein Family
UniProt Gene Name
IKZF2
UniProt Synonym Gene Names
HELIOS; ZNFN1A2
UniProt Entry Name
IKZF2_HUMAN
NCBI Protein Information
zinc finger protein Helios

NCBI Summary for IKZF2

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for IKZF2

Helios: Associates with Ikaros at centromeric heterochromatin. Belongs to the Ikaros C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; metal ion binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IKZF2 antibody

 Reference Product  PubMed Publications
 IKZF1 antibody  >9 publications with IKZF2 and IKZF1
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