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PPM1E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753165

Description: Rabbit IgG polyclonal antibody for PPM1E detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human.Background: protein phosphatase, Mg2+/Mn2+ dependent 1E is a protein that in humans is encoded by the PPM1E gene. It is mapped to 17q22.… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPM1E, Polyclonal Antibody
Full Product Name
Anti-PPM1E Antibody
Product Gene Name
Product Synonym Names
Protein phosphatase 1E; Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; PPM1E; CAMKN; KIAA1072; POPX1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for Q8WY54
Immunogen
E Coli-derived human PPM1E recombinant protein (Position: E141-E755).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PPM1E antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes for anti-PPM1E antibody
WB: 0.1-0.25ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Direct ELISA: 0.1-0.5ug/ml (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-PPM1E antibody

Rabbit Anti-PPM1E Polyclonal Antibody – Cat# MBS1753165

Immunofluorescence (IF) of anti-PPM1E antibody

Rabbit Anti-PPM1E Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753165

Immunohistochemistry (IHC) of anti-PPM1E antibody

Rabbit Anti-PPM1E Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753165

Western Blot (WB) of anti-PPM1E antibody

Figure 1. Western blot analysis of PPM1E using anti-PPM1E antibody.
Rabbit Anti-PPM1E Polyclonal Antibody, Western Blot (WB) – Cat# MBS1753165

Other Notes

Small volumes of anti-PPM1E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1E purchase

MBS1753165 is an antibody targeting PPM1E. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1E. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein phosphatase 1E
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1E
NCBI Official Symbol
PPM1E
NCBI Official Synonym Symbols
POPX1; PP2CH; caMKN; CaMKP-N
UniProt Protein Name
Protein phosphatase 1E
UniProt Synonym Protein Names
Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKP-N; CaMKP-nucleus; CaMKN; Partner of PIX 1; Partner of PIX-alpha
Protein Family
UniProt Gene Name
PPM1E
UniProt Synonym Gene Names
CAMKN; KIAA1072; POPX1; CaMKP-N; CaMKN
UniProt Entry Name
PPM1E_HUMAN
NCBI Protein Information
protein phosphatase 1E

NCBI Summary for PPM1E

This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
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UniProt Comments for PPM1E

PPM1E: Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Belongs to the PP2C family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: protein complex; mitochondrion; nucleolus; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: positive regulation of stress fiber formation; negative regulation of protein kinase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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