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HSPB8 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753236

Description: Mouse IgG monoclonal antibody for HSPB8/Hsp22 detection. Tested with WB, FCM in Human;Rat.Background: Heat shock protein beta-8 is a protein that in humans is encoded by the HSPB8 gene. The protein encoded by this gene belongs to the superfamily… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HSPB8/Hsp22, Monoclonal Antibody
Full Product Name
Anti-HSPB8/Hsp22 Antibody (Monoclonal, 7D8)
Product Gene Name
Product Synonym Names
CMT2L; CRYAC; DHMN 2; DHMN2; E2IG1; H11; HMN 2; HMN2; HMN2A; HSB8; HSPB 8; HspB8
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7D8
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
gene 608673
3D Structure
ModBase 3D Structure for Q9UJY1
Immunogen
E Coli-derived human HSPB8/Hsp22 recombinant protein (Position: M1-T196). Human HSPB8/Hsp22 shares 94.4% and 95.4% amino acid (aa) sequence identity with mouse and rat HSPB8/Hsp22, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HSPB8 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-HSPB8 antibody
WB: 0.1-0.5ug/ml (Human, Rat)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.

Product References and Citations for anti-HSPB8 antibody

1. Smith CC, Yu YX, Kulka M, Aurelian L (Sep 2000). "A novel human gene similar to the protein kinase (PK) coding domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) codes for a serine-threonine PK and is expressed in melanoma cells". J Biol Chem. 275 (33): 25690-9.
2. "Entrez Gene: HSPB8 Heat shock 22kDa protein 8".

Other Notes

Small volumes of anti-HSPB8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HSPB8 purchase

MBS1753236 is an antibody targeting HSPB8. Also known as Anti-HSPB8/Hsp22 Antibody (Monoclonal, 7D8). Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HSPB8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Heat shock protein beta-8
NCBI Official Synonym Full Names
heat shock protein family B (small) member 8
NCBI Official Symbol
HSPB8
NCBI Official Synonym Symbols
H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22
UniProt Protein Name
Heat shock protein beta-8
UniProt Synonym Protein Names
Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22
Protein Family
UniProt Gene Name
HSPB8
UniProt Synonym Gene Names
CRYAC; E2IG1; HSP22; HspB8
UniProt Entry Name
HSPB8_HUMAN
NCBI Protein Information
heat shock protein beta-8

NCBI Summary for HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
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UniProt Comments for HSPB8

HSPB8: Displays temperature-dependent chaperone activity. Monomer. Interacts with HSPB1. Interacts with DNAJB6. By 17-beta-estradiol. Predominantly expressed in skeletal muscle and heart. Belongs to the small heat shock protein (HSP20) family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; intracellular; nucleus

Molecular Function: identical protein binding; protein binding; protein kinase activity

Disease: Charcot-marie-tooth Disease, Axonal, Type 2l; Neuronopathy, Distal Hereditary Motor, Type Iia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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