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HMGB3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753238

Description: Mouse IgG monoclonal antibody for HMG4 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.Background: High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HMG4 (HMGB3), Monoclonal Antibody
Full Product Name
Anti-HMG4 Antibody (Monoclonal, 7G13)
Product Gene Name
Product Synonym Names
chromosomal protein, Nonhistone, HMG4 antibody; High mobility group (nonhistone chromosomal) protein 4 antibody; High mobility group box 3 antibody; High mobility group protein 2a antibody; High mobility group protein 4 antibody; High mobility group protein B3 antibody; High mobility group protein HMG4 antibody; HMG 4 antibody; HMG-2a antibody; HMG-4 antibody; HMG2A antibody; HMGB 3 antibody; HMGB3 antibody; HMGB3_HUMAN antibody; MGC90319 antibody; Non histone chromosomal protein antibody; Nonhistone chromosomal protein HMG4 antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7G13
Host
Mouse
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
gene 300915
3D Structure
ModBase 3D Structure for O15347
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4 (62-95aa EMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKR), identical to the related mouse and rat sequences.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-HMGB3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-HMGB3 antibody
WB: 0.1-0.5ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.

Flow Cytometry (FC/FACS) of anti-HMGB3 antibody

Mouse Anti-HMGB3 Monoclonal Antibody – Cat# MBS1753238

Immunofluorescence (IF) of anti-HMGB3 antibody

Mouse Anti-HMGB3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753238

Immunohistochemistry (IHC) of anti-HMGB3 antibody

Mouse Anti-HMGB3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753238

Western Blot (WB) of anti-HMGB3 antibody

Mouse Anti-HMGB3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS1753238

Product References and Citations for anti-HMGB3 antibody

1. "Entrez Gene: HMGB3 high-mobility group box 3".
2. Davis DL, Burch JB (1992). "Isolation of a chicken HMG2 cDNA clone and evidence for an HMG2-specific 3'-untranslated region.". Gene 113 (2): 251-6.
3. Vaccari T, Beltrame M, Ferrari S, Bianchi ME (Aug 1998). "Hmg4, a new member of the Hmg1/2 gene family".Genomics 49 (2): 247-52.

Other Notes

Small volumes of anti-HMGB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HMGB3 purchase

MBS1753238 is an antibody targeting HMGB3. Also known as Anti-HMG4 Antibody (Monoclonal, 7G13). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HMGB3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
High mobility group protein B3
NCBI Official Synonym Full Names
high mobility group box 3
NCBI Official Symbol
HMGB3
NCBI Official Synonym Symbols
HMG4; HMG-4; HMG2A; HMG-2a
UniProt Protein Name
High mobility group protein B3
UniProt Synonym Protein Names
High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4
UniProt Gene Name
HMGB3
UniProt Synonym Gene Names
HMG2A; HMG4; HMG-2a; HMG-4
UniProt Entry Name
HMGB3_HUMAN
NCBI Protein Information
high mobility group protein B3

NCBI Summary for HMGB3

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
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UniProt Comments for HMGB3

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HMGB3 antibody

 Reference Product  PubMed Publications
 HMGA1 antibody  >1 publications with HMGB3 and HMGA1
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