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WFS1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753427

Wolframin is a protein that in humans is encoded by the WFS1 gene. This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma… More details

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Product Overview

Online Datasheet in PDF
Product Name
WFS1, Polyclonal Antibody
Full Product Name
Anti-WFS1 Antibody
Product Gene Name
anti-WFS1 antibody
Product Synonym Names
WFS1; Wolframin; wolframin ER transmembrane glycoprotein
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for WFS1 detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
100,292 Da
OMIM
116400
3D Structure
ModBase 3D Structure for O76024
Immunogen
E Coli-derived human WFS1 recombinant protein (Position: A61-H313).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P) and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-WFS1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes for anti-WFS1 antibody
WB: 0.25-0.5ug/ml|Human, Monkey|
IHC-P: 2-5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|

Western Blot (WB) of anti-WFS1 antibody

Figure 1. Western blot analysis of WFS1 using anti-WFS1 antibody (MBS1753427).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates
Lane 2: human T-47D whole cell lysates
Lane 3: monkey lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-WFS1 antigen affinity purified polyclonal antibody (Catalog # MBS1753427) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for WFS1 at approximately 100KD. The expected band size for WFS1 is at 100KD.
Rabbit Anti-WFS1 Polyclonal Antibody – Cat# MBS1753427

Immunohistochemistry (IHC) of anti-WFS1 antibody

Figure 2. IHC analysis of WFS1 using anti-WFS1 antibody (MBS1753427).
WFS1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-WFS1 Antibody (MBS1753427) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.
Rabbit Anti-WFS1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753427

Immunofluorescence (IF) of anti-WFS1 antibody

Figure 3. IF analysis of WFS1 using anti- WFS1 antibody (MBS1753427).
WFS1 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- WFS1 Antibody (MBS1753427) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-WFS1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753427

Flow Cytometry (FC/FACS) of anti-WFS1 antibody

Figure 4. Flow Cytometry analysis of U20S cells using anti-WFS1 antibody (MBS1753427).
Overlay histogram showing U20S cells stained with MBS1753427 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WFS1 Antibody (MBS1753427, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-WFS1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753427

Product References and Citations for anti-WFS1 antibody

1. Berry, V., Gregory-Evans, C., Emmett, W., Waseem, N., Raby, J., Prescott, D., Moore, A. T., Bhattacharya, S. S. Wolfram gene (WFS1) mutation causes autosomal dominant congenital nuclear cataract in humans. Europ. J. Hum. Genet. 21: 1356-1360, 2013.
2. Bespalova, I. N., Van Camp, G., Bom, S. J. H., Brown, D. J., Cryns, K., DeWan, A. T., Erson, A. E., Flothmann, K., Kunst, H. P. M., Kurnool, P., Sivakumaran, T. A., Cremers, C. W. R. J., Leal, S. M., Burmeister, M., Lesperance, M. M. Mutations in the Wolfram syndrome 1 gene (WFS1) are a common cause of low frequency sensorineural hearing loss. Hum. Molec. Genet. 10: 2501-2508, 2001.
3. Colosimo, A., Guida, V., Rigoli, L., Di Bella, C., De Luca, A., Briuglia, S., Stuppia, L., Salpietro, D. C., Dallapiccolo, B. Molecular detection of novel WFS1 mutations in patients with Wolfram syndrome by a DHPLC-based assay. Hum. Mutat. 21: 622-629, 2003.

Other Notes

Small volumes of anti-WFS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WFS1 purchase

MBS1753427 is an antibody targeting WFS1. Reactive in Human, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WFS1. It may not necessarily be applicable to this product.

NCBI GI #
119602804
NCBI GeneID
7466
NCBI Accession #
EAW82398.1
UniProt Primary Accession #
O76024
UniProt Secondary Accession #
Q8N6I3; Q9UNW6; B2R797; D3DVT1
UniProt Related Accession #
O76024
NCBI Official Full Name
Wolfram syndrome 1 (wolframin), isoform CRA_a
NCBI Official Synonym Full Names
Wolfram syndrome 1 (wolframin)
NCBI Official Symbol
WFS1
NCBI Official Synonym Symbols
WFS; WFRS; WFSL; CTRCT41
UniProt Protein Name
Wolframin
Protein Family
Wolframin
UniProt Gene Name
WFS1
UniProt Entry Name
WFS1_HUMAN
NCBI Protein Information
wolframin

NCBI Summary for WFS1

This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
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UniProt Comments for WFS1

WFS1: Participates in the regulation of cellular Ca(2+) homeostasis, at least partly, by modulating the filling state of the endoplasmic reticulum Ca(2+) store. Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.

Protein type: Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; dendrite; integral to endoplasmic reticulum membrane

Molecular Function: calmodulin binding; transcription activator binding; transporter activity; ubiquitin protein ligase binding; calcium-dependent protein binding; ATPase binding

Biological Process: protein maturation via protein folding; ER-associated protein catabolic process; olfactory behavior; protein stabilization; positive regulation of proteolysis; unfolded protein response; negative regulation of transcription factor activity; glucose homeostasis; positive regulation of protein metabolic process; neurological system process; calcium ion homeostasis; endoplasmic reticulum calcium ion homeostasis; unfolded protein response, activation of signaling protein activity; sensory perception of sound; cellular protein metabolic process; visual perception; positive regulation of protein ubiquitination; negative regulation of programmed cell death; renal water homeostasis; negative regulation of neuron apoptosis; kidney development; positive regulation of calcium ion transport; positive regulation of growth; ER overload response

Disease: Deafness, Autosomal Dominant 6; Wolfram Syndrome 1; Wolfram-like Syndrome, Autosomal Dominant; Cataract 41; Diabetes Mellitus, Noninsulin-dependent
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Research Articles on WFS1

1. Identified a DNA substitution (c.1385A-to-G) in WFS1 exon.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WFS1 antibody

 Reference Product  PubMed Publications
 TCF7L2 antibody  >31 publications with WFS1 and TCF7L2
 CDKAL1 antibody  >26 publications with WFS1 and CDKAL1
 IGF2BP2 antibody  >26 publications with WFS1 and IGF2BP2
 SLC30A8 antibody  >25 publications with WFS1 and SLC30A8
 HHEX antibody  >23 publications with WFS1 and HHEX
 CISD2 antibody  >4 publications with WFS1 and CISD2
 SYVN1 antibody  >2 publications with WFS1 and SYVN1
 ATF6 antibody  >2 publications with WFS1 and ATF6

Pathways associated with anti-WFS1 antibody

 Products by Pathway  Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Unfolded Protein Response (UPR) Pathway antibodies  Unfolded Protein Response (UPR) Pathway Diagram
 XBP1(S) Activates Chaperone Genes Pathway antibodies  XBP1(S) Activates Chaperone Genes Pathway Diagram

Diseases associated with anti-WFS1 antibody

 Disease Name  Pubmed Publications
 Wolfram Syndrome Antibodies  >168 publications with WFS1 and Wolfram Syndrome
 Nervous System Diseases Antibodies  >135 publications with WFS1 and Nervous System Diseases
 Kidney Diseases Antibodies  >106 publications with WFS1 and Kidney Diseases
 Atrophy Antibodies  >96 publications with WFS1 and Atrophy
 Diabetes Mellitus, Type 2 Antibodies  >49 publications with WFS1 and Diabetes Mellitus, Type 2
 Mental Disorders Antibodies  >23 publications with WFS1 and Mental Disorders
 Mood Disorders Antibodies  >17 publications with WFS1 and Mood Disorders
 Insulin Resistance Antibodies  >13 publications with WFS1 and Insulin Resistance
 Disease Models, Animal Antibodies  >7 publications with WFS1 and Disease Models, Animal
 Glucose Intolerance Antibodies  >7 publications with WFS1 and Glucose Intolerance

Organs/Tissues associated with anti-WFS1 antibody

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >41 publications with WFS1 and Pancreas
 Brain Antibodies  >35 publications with WFS1 and Brain
 Nerve Antibodies  >19 publications with WFS1 and Nerve
 Eye Antibodies  >8 publications with WFS1 and Eye
 Liver Antibodies  >7 publications with WFS1 and Liver
 Kidney Antibodies  >5 publications with WFS1 and Kidney
 Bone Antibodies  >5 publications with WFS1 and Bone
 Connective Tissue Antibodies  >2 publications with WFS1 and Connective Tissue
 Skin Antibodies  >1 publications with WFS1 and Skin
 Ovary Antibodies  >1 publications with WFS1 and Ovary
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