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CDC25C Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753429

M-phase inducer phosphatase 3 is an enzyme that in humans is encoded by the CDC25C gene. This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cdc25c, Polyclonal Antibody
Full Product Name
Anti-Cdc25c Antibody
Product Gene Name
anti-Cdc25c antibody
Product Synonym Names
Cdc25c; Cdc25m1; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; cell division cycle 25C
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for Cdc25c detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
50,046 Da
3D Structure
ModBase 3D Structure for P48967
Immunogen
E Coli-derived mouse Cdc25c recombinant protein (Position: K24-Q447).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Cdc25c antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes for anti-Cdc25c antibody
WB: 0.25-0.5ug/ml|Rat|
Direct ELISA: 0.1-0.5ug/ml|Mouse|

Western Blot (WB) of anti-Cdc25c antibody

Figure 1. Western blot analysis of Cdc25c using anti-Cdc25c antibody (MBS1753429).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Cdc25c antigen affinity purified polyclonal antibody (Catalog # MBS1753429) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Cdc25c at approximately 53KD. The expected band size for Cdc25c is at 53KD.
Rabbit Anti-CDC25C Polyclonal Antibody – Cat# MBS1753429

Product References and Citations for anti-Cdc25c antibody

1. "Entrez Gene: CDC25C cell division cycle 25 homolog C (S. pombe)".
2. Gould KL, Moreno S, Tonks NK, Nurse P (Feb 1991). "Complementation of the mitotic activator, p80cdc25, by a human protein-tyrosine phosphatase".  Science 250 (4987): 1573-6.
3. Sartor H, Ehlert F, Grzeschik KH, et al. (1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively".  Genomics 13(3): 911-2.

Other Notes

Small volumes of anti-Cdc25c antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdc25c purchase

MBS1753429 is an antibody targeting Cdc25c. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cdc25c. It may not necessarily be applicable to this product.

NCBI GI #
122114545
NCBI GeneID
12532
NCBI Accession #
NP_033990.2
UniProt Primary Accession #
P48967
UniProt Secondary Accession #
Q99KG6
UniProt Related Accession #
P48967
GenBank Nucleotide #
NM_009860.2
NCBI Official Full Name
M-phase inducer phosphatase 3
NCBI Official Synonym Full Names
cell division cycle 25C
NCBI Official Symbol
Cdc25c
NCBI Official Synonym Symbols
Cdc25
UniProt Protein Name
M-phase inducer phosphatase 3
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25C
UniProt Gene Name
Cdc25c
UniProt Synonym Gene Names
Cdc25m1
UniProt Entry Name
MPIP3_MOUSE
NCBI Protein Information
M-phase inducer phosphatase 3; cell cycle phosphatase CDC25C; dual specificity phosphatase Cdc25C; cell division cycle control protein 25C

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Cdc25c antibody

 Reference Product  PubMed Publications
 Plk1 antibody  >57 publications with Cdc25c and Plk1

Pathways associated with anti-Cdc25c antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Signaling Pathway antibodies  Cell Cycle Signaling Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram

Diseases associated with anti-Cdc25c antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >328 publications with Cdc25c and Neoplasms
 Carcinoma Antibodies  >124 publications with Cdc25c and Carcinoma
 Breast Neoplasms Antibodies  >48 publications with Cdc25c and Breast Neoplasms
 Leukemia Antibodies  >43 publications with Cdc25c and Leukemia
 Adenocarcinoma Antibodies  >40 publications with Cdc25c and Adenocarcinoma
 Drug Toxicity Antibodies  >36 publications with Cdc25c and Drug Toxicity
 Lung Neoplasms Antibodies  >35 publications with Cdc25c and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >35 publications with Cdc25c and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >31 publications with Cdc25c and Neoplasms, Experimental
 Colorectal Neoplasms Antibodies  >28 publications with Cdc25c and Colorectal Neoplasms

Organs/Tissues associated with anti-Cdc25c antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >50 publications with Cdc25c and Lung
 Brain Antibodies  >18 publications with Cdc25c and Brain
 Skin Antibodies  >15 publications with Cdc25c and Skin
 Bone Antibodies  >15 publications with Cdc25c and Bone
 Stomach Antibodies  >14 publications with Cdc25c and Stomach
 Testis Antibodies  >9 publications with Cdc25c and Testis
 Kidney Antibodies  >6 publications with Cdc25c and Kidney
 Embryonic Tissue Antibodies  >6 publications with Cdc25c and Embryonic Tissue
 Bone Marrow Antibodies  >5 publications with Cdc25c and Bone Marrow
 Intestine Antibodies  >5 publications with Cdc25c and Intestine
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