DLL1 Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of DLL1 using anti-DLL1 antibody (MBS1753531).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HELA whole cell lysates
Lane 2: monkey COS-7 whole cell lysates
Lane 3: huamn Jurkat whole cell lysates
Lane 4: huamn Raji whole cell lysates
Lane 5: human SW620 whole cell lysates
Lane 6: human HepG2 whole cell lysates
Lane 7: human U20S whole cell lysates
Lane 8: rat heart tissue lysates
Lane 9: rat C6 whole cell lysates
Lane 10: mouse heart tissue lysates
Lane 11: mouse Neuro-2a whole cell lysates
Lane 12: mouse RAW264. 7 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DLL1 antigen affinity purified polyclonal antibody (Catalog # MBS1753531) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for DLL1 at approximately 78KD. The expected band size for DLL1 is at 78KD.

Figure 2. IHC analysis of DLL1 using anti-DLL1 antibody (MBS1753531).
DLL1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DLL1 Antibody (MBS1753531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.

Figure 3. IF analysis of DLL1 using anti-DLL1 antibody (MBS1753531).
DLL1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-DLL1 Antibody (MBS1753531) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 4. Flow Cytometry analysis of THP-1 cells using anti-DLL1 antibody (MBS1753531).
Overlay histogram showing THP-1 cells stained with MBS1753531 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DLL1 Antibody (MBS1753531,1μg/1x10⁶ cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.