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Rnase L/Rnasel (RNASEL) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753534

RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated… More details

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Product Overview

Online Datasheet in PDF
Product Name
RNase L/Rnasel, Polyclonal Antibody
Full Product Name
Anti-RNase L/Rnasel Antibody
Product Gene Name
anti-Rnasel antibody
Product Synonym Names
Rnasel; Rns4; 2-5A-dependent ribonuclease; 2-5A-dependent RNase; EC 3. 1. 26. -; Ribonuclease 4; Ribonuclease L; RNase L; ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent)
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for RNase L/Rnasel detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
83,275 Da
3D Structure
ModBase 3D Structure for Q05921
Immunogen
E Coli-derived mouse RNase L/Rnasel recombinant protein (Position: E24-S735).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-Rnasel antibody
Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes for anti-Rnasel antibody
WB: 0.25-0.5ug/ml|Mouse|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Mouse|

Western Blot (WB) of anti-Rnasel antibody

Figure 1. Western blot analysis of RNase L/Rnasel using anti-RNase L/Rnasel antibody (MBS1753534).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates
Lane 2: mouse Raw264. 7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RNase L/Rnasel antigen affinity purified polyclonal antibody (Catalog # MBS1753534) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for RNase L/Rnasel at approximately 83KD. The expected band size for RNase L/Rnasel is at 83KD.
Rabbit Anti-RNASEL Polyclonal Antibody – Cat# MBS1753534

Flow Cytometry (FC/FACS) of anti-Rnasel antibody

Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-RNase L/Rnasel antibody (MBS1753534).
Overlay histogram showing HEPA1-6 cells stained with MBS1753534 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNase L/Rnasel Antibody (MBS1753534, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-RNASEL Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753534

Flow Cytometry (FC/FACS) of anti-Rnasel antibody

Figure 3. Flow Cytometry analysis of NRK cells using anti-RNase L/Rnasel antibody (MBS1753534).
Overlay histogram showing NRK cells stained with MBS1753534 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNase L/Rnasel Antibody (MBS1753534, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-RNASEL Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753534

Product References and Citations for anti-Rnasel antibody

1. RNase L inhibitor: a new component of the interferon-regulated 2-5A pathway. J. Biol. Chem. 270: 13308-13317, 1995.
2. Carpten, J., Nupponen, N., Isaacs, S., Sood, R., Robbins, C., Xu, J., Faruque, M., Moses, T., Ewing, C., Gillanders, E., Hu, P., Bujnovszky, P., and 29 others. Germline mutations in the ribonuclease L gene in families showing linkage with HPC1. Nature Genet. 30: 181-184, 2002.
3. Casey, G., Neville, P. J., Plummer, S. J., Xiang, Y., Krumroy, L. M., Klein, E. A., Catalona, W. J., Nupponen, N., Carpten, J. D., Trent, J. M., Silverman, R. H., Witte, J. S. RNASEL arg462gln variant is implicated in up to 13% of prostate cancer cases. Nature Genet. 32: 581-583, 2002.

Other Notes

Small volumes of anti-Rnasel antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rnasel purchase

MBS1753534 is an antibody targeting Rnasel. Also known as Anti-RNase L/Rnasel Antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rnasel. It may not necessarily be applicable to this product.

NCBI GI #
31982957
NCBI GeneID
24014
NCBI Accession #
NP_036012.1
UniProt Primary Accession #
Q05921
UniProt Secondary Accession #
Q9ERU7
UniProt Related Accession #
Q05921
GenBank Nucleotide #
NM_011882.2
NCBI Official Full Name
2-5A-dependent ribonuclease
NCBI Official Synonym Full Names
ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent)
NCBI Official Symbol
Rnasel
NCBI Official Synonym Symbols
E230029I04Rik
UniProt Protein Name
2-5A-dependent ribonuclease
UniProt Synonym Protein Names
Ribonuclease 4; Ribonuclease L; RNase L
Protein Family
2-5A-dependent ribonuclease
UniProt Gene Name
Rnasel
UniProt Synonym Gene Names
Rns4; 2-5A-dependent RNase; RNase L
NCBI Protein Information
2-5A-dependent ribonuclease

UniProt Comments for Rnasel

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis. Might play a central role in the regulation of mRNA turnover (). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length ().
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Research Articles on Rnasel

1. Pref-1 mRNA is a novel substrate of RNase-L.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Rnasel antibody

 Products by Pathway  Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with anti-Rnasel antibody

 Disease Name  Pubmed Publications
 Prostatic Diseases Antibodies  >73 publications with Rnasel and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >73 publications with Rnasel and Prostatic Neoplasms
 Inflammation Antibodies  >15 publications with Rnasel and Inflammation
 Necrosis Antibodies  >4 publications with Rnasel and Necrosis
 Musculoskeletal Diseases Antibodies  >3 publications with Rnasel and Musculoskeletal Diseases
 Liver Diseases Antibodies  >2 publications with Rnasel and Liver Diseases
 Disease Models, Animal Antibodies  >2 publications with Rnasel and Disease Models, Animal
 Hyperplasia Antibodies  >2 publications with Rnasel and Hyperplasia
 Neoplasm Metastasis Antibodies  >2 publications with Rnasel and Neoplasm Metastasis
 Atrophy Antibodies  >1 publications with Rnasel and Atrophy

Organs/Tissues associated with anti-Rnasel antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >16 publications with Rnasel and Blood
 Lung Antibodies  >3 publications with Rnasel and Lung
 Kidney Antibodies  >3 publications with Rnasel and Kidney
 Muscle Antibodies  >2 publications with Rnasel and Muscle
 Skin Antibodies  >2 publications with Rnasel and Skin
 Brain Antibodies  >2 publications with Rnasel and Brain
 Bladder Antibodies  >1 publications with Rnasel and Bladder
 Bone Marrow Antibodies  >1 publications with Rnasel and Bone Marrow
 Lymph Node Antibodies  >1 publications with Rnasel and Lymph Node
 Embryonic Tissue Antibodies  >1 publications with Rnasel and Embryonic Tissue
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