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FOXJ1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753681

Forkhead box protein J1 is a protein that in humans is encoded by the FOXJ1 gene. This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that… More details

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Product Overview

Online Datasheet in PDF
Product Name
FOXJ1, Polyclonal Antibody
Full Product Name
Anti-FOXJ1 Antibody
Product Gene Name
anti-FOXJ1 antibody
Product Synonym Names
FOXJ1; FKHL13; HFH4; Forkhead box protein J1; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; forkhead box J1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for FOXJ1 detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
45,247 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (74132414..74137380, complement). Location: 17q25.1
OMIM
602291
3D Structure
ModBase 3D Structure for Q92949
Immunogen
E Coli-derived human FOXJ1 recombinant protein (Position: D27-A405).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P) and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FOXJ1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes for anti-FOXJ1 antibody
WB: 0.25-0.5ug/ml|Human, Rat|
IHC-P: 2-5ug/ml|Rat|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|

Immunohistochemistry (IHC) of anti-FOXJ1 antibody

Figure 2. IHC analysis of FOXJ1 using anti-FOXJ1 antibody (MBS1753681).
FOXJ1 was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FOXJ1 Antibody (MBS1753681) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.
Rabbit Anti-FOXJ1 Polyclonal Antibody – Cat# MBS1753681

Immunofluorescence (IF) of anti-FOXJ1 antibody

Figure 3. IF analysis of FOXJ1 using anti- FOXJ1 antibody (MBS1753681).
FOXJ1 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- FOXJ1 Antibody (MBS1753681) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-FOXJ1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753681

Flow Cytometry (FC/FACS) of anti-FOXJ1 antibody

Figure 4. Flow Cytometry analysis of HepG2 cells using anti-FOXJ1 antibody (MBS1753681).
Overlay histogram showing HepG2 cells stained with MBS1753681 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXJ1 Antibody (MBS1753681, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-FOXJ1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753681

Product References and Citations for anti-FOXJ1 antibody

1. Avraham, K. B., Fletcher, C., Overdier, D. G., Clevidence, D. E., Lai, E., Costa, R. H., Jenkins, N. A., Copeland, N. G. Murine chromosomal location of eight members of the hepatocyte nuclear factor 3/fork head winged helix family of transcription factors. Genomics 25: 388-393, 1995.
2. Chen, J., Knowles, H. J., Hebert, J. L., Hackett, B. P. Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene results in an absence of cilia and random left-right asymmetry. J. Clin. Invest. 102: 1077-1082, 1998.
3. Li, C. -S., Chae, S. -C., Lee, J. -H., Zhang, Q., Chung, H. -T. Identification of single nucleotide polymorphisms in FOXJ1 and their association with allergic rhinitis. J. Hum. Genet. 51: 292-297, 2006.

Other Notes

Small volumes of anti-FOXJ1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FOXJ1 purchase

MBS1753681 is an antibody targeting FOXJ1. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FOXJ1. It may not necessarily be applicable to this product.

NCBI GI #
50301236
NCBI GeneID
2302
NCBI Accession #
NP_001445.2
UniProt Primary Accession #
Q92949
UniProt Secondary Accession #
O00630
UniProt Related Accession #
Q92949
GenBank Nucleotide #
NM_001454.3
NCBI Official Full Name
forkhead box protein J1
NCBI Official Synonym Full Names
forkhead box J1
NCBI Official Symbol
FOXJ1
NCBI Official Synonym Symbols
HFH4; HFH-4; FKHL13
UniProt Protein Name
Forkhead box protein J1
UniProt Synonym Protein Names
Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4
Protein Family
Forkhead box protein
UniProt Gene Name
FOXJ1
UniProt Synonym Gene Names
FKHL13; HFH4; HFH-4
UniProt Entry Name
FOXJ1_HUMAN
NCBI Protein Information
forkhead box protein J1; forkhead-like 13; fork head homologue 4; forkhead-related protein FKHL13; forkhead transcription factor HFH-4; hepatocyte nuclear factor 3 forkhead homolog 4

NCBI Summary for FOXJ1

This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.[provided by RefSeq, Sep 2009]
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UniProt Comments for FOXJ1

FOXJ1: May play an important role in cell fate determination during lung development and in spermatogenesis. Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of germinal center formation; positive regulation of central B cell tolerance induction; establishment of apical/basal cell polarity; negative regulation of B cell activation; negative regulation of interleukin-6 biosynthetic process; negative regulation of T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; humoral immune response; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of T cell proliferation; inhibition of NF-kappaB transcription factor; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; brain development; actin cytoskeleton organization and biogenesis; leukocyte migration; central tolerance induction

Disease: Allergic Rhinitis
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Research Articles on FOXJ1

1. Rer1p depletion reduced ciliary length and function by increasing gamma-secretase complex assembly and activity and, consequently, enhancing Notch signaling as well as reducing Foxj1a expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-FOXJ1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with FOXJ1 and Inflammation
 Neoplasms Antibodies  >6 publications with FOXJ1 and Neoplasms
 Congenital Abnormalities Antibodies  >6 publications with FOXJ1 and Congenital Abnormalities
 Cardiovascular Diseases Antibodies  >5 publications with FOXJ1 and Cardiovascular Diseases
 Fibrosis Antibodies  >4 publications with FOXJ1 and Fibrosis
 Heart Defects, Congenital Antibodies  >4 publications with FOXJ1 and Heart Defects, Congenital
 Disease Models, Animal Antibodies  >4 publications with FOXJ1 and Disease Models, Animal
 Drug Toxicity Antibodies  >3 publications with FOXJ1 and Drug Toxicity
 Hyperplasia Antibodies  >3 publications with FOXJ1 and Hyperplasia
 Carcinoma, Hepatocellular Antibodies  >2 publications with FOXJ1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-FOXJ1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >48 publications with FOXJ1 and Lung
 Embryonic Tissue Antibodies  >26 publications with FOXJ1 and Embryonic Tissue
 Brain Antibodies  >19 publications with FOXJ1 and Brain
 Kidney Antibodies  >6 publications with FOXJ1 and Kidney
 Testis Antibodies  >4 publications with FOXJ1 and Testis
 Pancreas Antibodies  >2 publications with FOXJ1 and Pancreas
 Connective Tissue Antibodies  >1 publications with FOXJ1 and Connective Tissue
 Ovary Antibodies  >1 publications with FOXJ1 and Ovary
 Uterus Antibodies  >1 publications with FOXJ1 and Uterus
 Uncharacterized Tissue Antibodies  >1 publications with FOXJ1 and Uncharacterized Tissue
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