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BDH1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753800

D-beta-hydroxybutyrate dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the BDH1 gene. This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme… More details

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Product Overview

Online Datasheet in PDF
Product Name
BDH1, Polyclonal Antibody
Full Product Name
Anti-BDH1 Antibody
Product Gene Name
anti-BDH1 antibody
Product Synonym Names
BDH1; BDH; SDR9C1; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; EC 1. 1. 1. 30; 3-hydroxybutyrate dehydrogenase; BDH; Short chain dehydrogenase/reductase family 9C member 1; 3-hydroxybutyrate dehydrogenase 1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for BDH1 detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
343
OMIM
603063
3D Structure
ModBase 3D Structure for Q02338
Immunogen
E Coli-derived human BDH1 recombinant protein (Position: M1-Y287).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC(P) and ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-BDH1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
Application Notes for anti-BDH1 antibody
WB: 0.25-0.5ug/ml|Human, Rat|
IHC-P: 2-5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|

Western Blot (WB) of anti-BDH1 antibody

Figure 1. Western blot analysis of BDH1 using anti-BDH1 antibody (MBS1753800).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates
Lane 2: human COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-BDH1 antigen affinity purified polyclonal antibody (Catalog # MBS1753800) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for BDH1 at approximately 31KD. The expected band size for BDH1 is at 38KD.
Rabbit Anti-BDH1 Polyclonal Antibody – Cat# MBS1753800

Immunohistochemistry (IHC) of anti-BDH1 antibody

Figure 2. IHC analysis of BDH1 using anti-BDH1 antibody (MBS1753800).
BDH1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BDH1 Antibody (MBS1753800) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.
Rabbit Anti-BDH1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753800

Immunohistochemistry (IHC) of anti-BDH1 antibody

Figure 3. IHC analysis of BDH1 using anti-BDH1 antibody (MBS1753800).
BDH1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BDH1 Antibody (MBS1753800) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.
Rabbit Anti-BDH1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753800

Immunohistochemistry (IHC) of anti-BDH1 antibody

Figure 4. IHC analysis of BDH1 using anti-BDH1 antibody (MBS1753800).
BDH1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BDH1 Antibody (MBS1753800) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen.
Rabbit Anti-BDH1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS1753800

Immunofluorescence (IF) of anti-BDH1 antibody

Figure 5. IF analysis of BDH1 using anti-BDH1 antibody (MBS1753800).
BDH1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-BDH1 Antibody (MBS1753800) overnight at 4 degree C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-BDH1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1753800

Flow Cytometry (FC/FACS) of anti-BDH1 antibody

Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (MBS1753800).
Overlay histogram showing U937 cells stained with MBS1753800 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (MBS1753800,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-BDH1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1753800

Product References and Citations for anti-BDH1 antibody

1. Marks, A. R., McIntyre, J. O., Duncan, T. M., Erdjument-Bromage, H., Tempst, P., Fleischer, S. Molecular cloning and characterization of (R)-3-hydroxybutyrate dehydrogenase from human heart. J. Biol. Chem. 267: 15459-15463, 1992.
2. Persson, B., Kallberg, Y., Bray, J. E., Bruford, E., Dellaporta, S. L., Favia, A. D., Duarte, R. G., Jornvall, H., Kavanagh, K. L., Kedishvili, N., Kisiela, M., Maser, E., Mindnich, R., Orchard, S., Penning, T. M., Thornton, J. M., Adamski, J., Oppermann, U. The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative. Chem. Biol. Interact. 178: 94-98, 2009.

Other Notes

Small volumes of anti-BDH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BDH1 purchase

MBS1753800 is an antibody targeting BDH1. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BDH1. It may not necessarily be applicable to this product.

NCBI GI #
25108876
NCBI GeneID
622
NCBI Accession #
Q02338.3
UniProt Primary Accession #
Q02338
UniProt Secondary Accession #
Q96ET1; Q9BRZ4; D3DXC0
UniProt Related Accession #
Q02338
NCBI Official Full Name
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
NCBI Official Synonym Full Names
3-hydroxybutyrate dehydrogenase, type 1
NCBI Official Symbol
BDH1
NCBI Official Synonym Symbols
BDH; SDR9C1
UniProt Protein Name
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
UniProt Synonym Protein Names
3-hydroxybutyrate dehydrogenase
Protein Family
(R,R)-butanediol dehydrogenase
UniProt Gene Name
BDH1
UniProt Synonym Gene Names
BDH; BDH
UniProt Entry Name
BDH_HUMAN
NCBI Protein Information
D-beta-hydroxybutyrate dehydrogenase, mitochondrial; (R)-3-hydroxybutyrate dehydrogenase; 3-hydroxybutyrate dehydrogenase (heart, mitochondrial); short chain dehydrogenase/reductase family 9C, member 1

NCBI Summary for BDH1

This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for BDH1

BDH: a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - butanoate; Oxidoreductase; EC 1.1.1.30; Lipid Metabolism - synthesis and degradation of ketone bodies; Mitochondrial

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane

Molecular Function: 3-hydroxybutyrate dehydrogenase activity; phospholipid binding

Biological Process: response to drug; response to toxin; cellular lipid metabolic process; ketone body metabolic process; liver development; response to estradiol stimulus; response to insulin stimulus; response to starvation; response to cadmium ion; response to ethanol; ketone body catabolic process; ketone body biosynthetic process; brain development; response to nutrient; response to corticosterone stimulus
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Research Articles on BDH1

1. Data show that the ketone body metabolizing enzymes BDH1, BDH2, OXCT1 and ACAT1 were expressed at the mRNA and protein level in all glioma cell lines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BDH1 antibody

 Reference Product  PubMed Publications
 BDH2 antibody  >5 publications with BDH1 and BDH2
 OXCT1 antibody  >4 publications with BDH1 and OXCT1
 HMGCL antibody  >3 publications with BDH1 and HMGCL

Pathways associated with anti-BDH1 antibody

 Products by Pathway  Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway antibodies  Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway Diagram
 Ketone Body Metabolism Pathway antibodies  Ketone Body Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Synthesis And Degradation Of Ketone Bodies Pathway antibodies  Synthesis And Degradation Of Ketone Bodies Pathway Diagram
 Synthesis And Degradation Of Ketone Bodies Pathway antibodies  Synthesis And Degradation Of Ketone Bodies Pathway Diagram

Diseases associated with anti-BDH1 antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >4 publications with BDH1 and Fatty Liver
 Breast Neoplasms Antibodies  >2 publications with BDH1 and Breast Neoplasms
 Inflammation Antibodies  >2 publications with BDH1 and Inflammation
 Anorexia Antibodies  >1 publications with BDH1 and Anorexia
 Cardiovascular Diseases Antibodies  >1 publications with BDH1 and Cardiovascular Diseases
 Weight Loss Antibodies  >1 publications with BDH1 and Weight Loss
 Neoplasm Metastasis Antibodies  >1 publications with BDH1 and Neoplasm Metastasis
 Heart Diseases Antibodies  >1 publications with BDH1 and Heart Diseases

Organs/Tissues associated with anti-BDH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with BDH1 and Blood
 Liver Antibodies  >5 publications with BDH1 and Liver
 Heart Antibodies  >3 publications with BDH1 and Heart
 Muscle Antibodies  >2 publications with BDH1 and Muscle
 Brain Antibodies  >2 publications with BDH1 and Brain
 Adipose Tissue Antibodies  >1 publications with BDH1 and Adipose Tissue
 Prostate Antibodies  >1 publications with BDH1 and Prostate
 Mammary Gland Antibodies  >1 publications with BDH1 and Mammary Gland
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