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IL3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175381

Description: Rabbit IgG polyclonal antibody for Interleukin-3(IL3) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Human.Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL3, Polyclonal Antibody
Full Product Name
Anti-human IL3 antibody
Product Gene Name
anti-IL3 antibody
Product Synonym Names
Interleukin-3(IL-3); interleukin 3(colony-stimulating factor, multiple); Burst promoting activity antibody; Colony stimulating factor multiple antibody; Eosinophil colony stimulating factor antibody; Hematopoietic growth factor antibody; IL 3 antibody; IL-3 antibody; IL3 antibody; Interleukin 3 (colony stimulating factor, multiple) antibody; Interleukin 3 antibody; Interleukin-3 antibody; Mast cell growth factor antibody; MCGF antibody; MGC79398 antibody; MGC79399 antibody; Multi CSF antibody; Multilineage colony stimulating factor antibody; Multipotential colony stimulating factor antibody; Multipotential colony-stimulating factor antibody; OTTHUMP00000065963 antibody; P cell stimulating factor antibody; P-cell-stimulating factor antibody; Thy1 inducing factor antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
17,233 Da
OMIM
147740
3D Structure
ModBase 3D Structure for P08700
Immunogen
E Coli-derived human IL-3 recombinant protein (Position: A20-F152).
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IL3 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Neu, Immunoprecipitation (IP)

Western Blot (WB) of anti-IL3 antibody

Figure. Western blot analysis of IL-3 using anti- IL-3 antibody (MBS175381).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane : Recombinant Human IL-3 Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-3 at approximately 15KD. The expected band size for IL-3 is at 15KD.
Rabbit Anti-IL3 Polyclonal Antibody – Cat# MBS175381

Other Notes

Small volumes of anti-IL3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL3 purchase

MBS175381 is an antibody targeting IL3. Also known as Anti-human IL3 antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL3. It may not necessarily be applicable to this product.

NCBI GI #
124330
NCBI GeneID
3562
NCBI Accession #
P08700.2
UniProt Primary Accession #
P08700
UniProt Secondary Accession #
Q6GS87
UniProt Related Accession #
P08700
NCBI Official Full Name
Interleukin-3
NCBI Official Synonym Full Names
interleukin 3
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MULTI-CSF
UniProt Protein Name
Interleukin-3
UniProt Synonym Protein Names
Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
Protein Family
Interleukin
UniProt Gene Name
IL3
UniProt Synonym Gene Names
IL-3; MCGF
UniProt Entry Name
IL3_HUMAN
NCBI Protein Information
interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; colony-stimulating factor, multiple; multilineage-colony-stimulating factor; multipotential colony-stimulating factor

NCBI Summary for IL3

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL3

IL3: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. Belongs to the IL-3 family.

Protein type: Cell cycle regulation; Secreted, signal peptide; Secreted; Cytokine; Oncoprotein; Apoptosis

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-3 receptor binding; growth factor activity; cytokine activity

Biological Process: nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of tyrosine phosphorylation of Stat5 protein; embryonic hemopoiesis; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; immune response; positive regulation of myeloid leukocyte differentiation; positive regulation of DNA replication
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Research Articles on IL3

1. A single nucleotide polymorphism (SNP; rs20541) in the IL-13 gene has been recognized as a risk factor for asthma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IL3 antibody

 Reference Product  PubMed Publications
 IL2 antibody  >84 publications with IL3 and IL2
 IL5 antibody  >50 publications with IL3 and IL5
 JAK2 antibody  >13 publications with IL3 and JAK2
 IL7 antibody  >10 publications with IL3 and IL7
 CSF2RB antibody  >10 publications with IL3 and CSF2RB
 IL3RA antibody  >8 publications with IL3 and IL3RA
 FOS antibody  >7 publications with IL3 and FOS
 GRB2 antibody  >2 publications with IL3 and GRB2
 PIK3CA antibody  >1 publications with IL3 and PIK3CA

Pathways associated with anti-IL3 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-IL3 antibody

 Disease Name  Pubmed Publications
 Bone Marrow Diseases Antibodies  >42 publications with IL3 and Bone Marrow Diseases
 Necrosis Antibodies  >27 publications with IL3 and Necrosis
 Lung Diseases Antibodies  >24 publications with IL3 and Lung Diseases
 Inflammation Antibodies  >19 publications with IL3 and Inflammation
 Asthma Antibodies  >17 publications with IL3 and Asthma
 Anemia Antibodies  >12 publications with IL3 and Anemia
 Nervous System Diseases Antibodies  >10 publications with IL3 and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with IL3 and Brain Diseases
 Kidney Diseases Antibodies  >6 publications with IL3 and Kidney Diseases
 Lung Neoplasms Antibodies  >5 publications with IL3 and Lung Neoplasms

Organs/Tissues associated with anti-IL3 antibody

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >12 publications with IL3 and Mixed
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