NDUFB3 Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of NDUFB3 using anti-NDUFB3 antibody (MBS1753829).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-NDUFB3 antigen affinity purified polyclonal antibody (Catalog # MBS1753829) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for NDUFB3 at approximately 14KD. The expected band size for NDUFB3 is at 14KD.

Figure 2. Flow Cytometry analysis of A431 cells using anti-NDUFB3 antibody (MBS1753829).
Overlay histogram showing A431 cells stained with MBS1753829 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB3 Antibody (MBS1753829, 1μg/1x10⁶ cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.