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Alpha Amylase 1 (AMY1A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175466

Description: Rabbit IgG polyclonal antibody for Alpha-amylase 1(AMY1A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.Background: Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylase is present in human saliva, where it… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Alpha Amylase 1 (AMY1A), Polyclonal Antibody
Full Product Name
Anti-Alpha Amylase 1 antibody
Product Gene Name
Product Synonym Names
Alpha-amylase 1; amylase, alpha 1A(salivary); 1 antibody; 4-alpha-D-glucan glucanohydrolase 1 antibody; Alpha-amylase 1 antibody; AMY1_HUMAN antibody; AMY1A antibody; AMY1B antibody; AMY1C antibody; Salivary alpha-amylase antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
OMIM
gene 104702
3D Structure
ModBase 3D Structure for P04745
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha Amylase 1 (30-44aa HLFEWRWVDIALECE), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AMY1A antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-AMY1A antibody
WB: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
IHC-P: 0.5-1ug/ml; Tested Species: Rat; Predicted Species: Rat; Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for product based on sequence similarties.
By Heat: Boiling paraffin section in 10mM citrate buffer, pH6, for 20 mins is required for staining of formalin/paraffin sections.

Other application have not been tested.
Optimal dilutions should be determined by end user.

Western Blot (WB) of anti-AMY1A antibody

Anti-Alpha Amylase 1 antibody, MBS175466, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Intestine Tissue Lysate
Rabbit Anti-AMY1A Polyclonal Antibody – Cat# MBS175466

Immunohistochemistry (IHC) of anti-AMY1A antibody

Anti-Alpha Amylase 1 antibody, MBS175466, IHC(P)
IHC(P): Rat Spleen Tissue
Rabbit Anti-AMY1A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175466

Other Notes

Small volumes of anti-AMY1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMY1A purchase

MBS175466 is an antibody targeting AMY1A. Also known as Anti-Alpha Amylase 1 antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AMY1A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Alpha-amylase 1
NCBI Official Synonym Full Names
amylase, alpha 1B (salivary)
NCBI Official Symbol
AMY1B
NCBI Official Synonym Symbols
AMY1
UniProt Protein Name
Alpha-amylase 1
UniProt Synonym Protein Names
1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase
Protein Family
UniProt Gene Name
AMY1A
UniProt Synonym Gene Names
AMY1
UniProt Entry Name
AMY1_HUMAN
NCBI Protein Information
alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1

NCBI Summary for AMY1A

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008]
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UniProt Comments for AMY1A

AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family.

Protein type: Hydrolase; Carbohydrate Metabolism - starch and sucrose; Secreted; EC 3.2.1.1; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular space

Molecular Function: alpha-amylase activity; protein binding; metal ion binding

Biological Process: carbohydrate metabolic process; digestion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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