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RPLP0 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755346

60S acidic ribosomal protein P0 is a protein that in humans is encoded by the RPLP0 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species… More details

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Product Overview

Online Datasheet in PDF
Product Name
RPLP0, Polyclonal Antibody
Full Product Name
Anti-RPLP0 Antibody Picoband
Product Gene Name
anti-RPLP0 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
27,436 Da
OMIM
180510
3D Structure
ModBase 3D Structure for P05388
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human RPLP0 recombinant protein (Position: M1-D271).

Product Categories/Family for anti-RPLP0 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RPLP0 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RPLP0 antibody
WB: 0.1-0.25 ug/ml, Human
ICC/IF: 5 ug/ml, Human
FC/FACS: 1-3 ug/1x10^6 cells, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (#MBS176453) for ICC.

Western Blot (WB) of anti-RPLP0 antibody

Figure 1. Western blot analysis of RPLP0 using anti-RPLP0 antibody (MBS1755346).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human A375 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (#MBS1755346) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 34 kDa.
Rabbit Anti-RPLP0 Polyclonal Antibody – Cat# MBS1755346

Immunofluorescence (IF) of anti-RPLP0 antibody

Figure 2. IF analysis of RPLP0 using anti-RPLP0 antibody (MBS1755346).

RPLP0 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-RPLP0 Antibody (MBS1755346) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG (MBS177405) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-RPLP0 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1755346

Flow Cytometry (FC/FACS) of anti-RPLP0 antibody

Figure 3. Flow Cytometry analysis of HL-60 cells using anti-RPLP0 antibody (MBS1755346).

Overlay histogram showing HL-60 cells stained with MBS1755346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPLP0 Antibody (MBS1755346, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-RPLP0 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1755346

Other Notes

Small volumes of anti-RPLP0 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPLP0 purchase

MBS1755346 is an antibody targeting RPLP0. Also known as Anti-RPLP0 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RPLP0. It may not necessarily be applicable to this product.

NCBI GI #
4506667
NCBI GeneID
6175
NCBI Accession #
NP_000993.1
UniProt Primary Accession #
P05388
UniProt Secondary Accession #
Q3B7A4; Q9BVK4
UniProt Related Accession #
P05388
GenBank Nucleotide #
NM_001002.3
NCBI Official Full Name
60S acidic ribosomal protein P0
NCBI Official Synonym Full Names
ribosomal protein, large, P0
NCBI Official Symbol
RPLP0
NCBI Official Synonym Symbols
P0; LP0; L10E; RPP0; PRLP0
UniProt Protein Name
60S acidic ribosomal protein P0
UniProt Synonym Protein Names
60S ribosomal protein L10E
UniProt Gene Name
RPLP0
UniProt Entry Name
RLA0_HUMAN
NCBI Protein Information
60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0

NCBI Summary for RPLP0

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPLP0

RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: focal adhesion; membrane; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; ribosome biogenesis and assembly; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination
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Research Articles on RPLP0

1. Evaluated autoantibodies against native ribosomal P complex and recombinant ribosomal P proteins (anti-Rib-P0, anti-Rib-P1, anti-Rib-P2) for prevalence, diagnostic relevance&clinical associations in a Chinese cohort with systemic lupus erythematosus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPLP0 antibody

 Reference Product  PubMed Publications
 RPLP2 antibody  >5 publications with RPLP0 and RPLP2
 RPL4 antibody  >4 publications with RPLP0 and RPL4
 RPL9 antibody  >2 publications with RPLP0 and RPL9
 RPL12 antibody  >1 publications with RPLP0 and RPL12
 RPS20 antibody  >1 publications with RPLP0 and RPS20
 RPL11 antibody  >1 publications with RPLP0 and RPL11
 RPS3 antibody  >1 publications with RPLP0 and RPS3

Pathways associated with anti-RPLP0 antibody

 Products by Pathway  Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytoplasmic Ribosomal Proteins Pathway antibodies  Cytoplasmic Ribosomal Proteins Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Eukaryotic Translation Termination Pathway antibodies  Eukaryotic Translation Termination Pathway Diagram
 Formation Of A Pool Of Free 40S Subunits Pathway antibodies  Formation Of A Pool Of Free 40S Subunits Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Influenza Infection Pathway antibodies  Influenza Infection Pathway Diagram

Diseases associated with anti-RPLP0 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >15 publications with RPLP0 and Carcinoma
 Breast Neoplasms Antibodies  >8 publications with RPLP0 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >5 publications with RPLP0 and Neoplasms, Experimental
 Inflammation Antibodies  >4 publications with RPLP0 and Inflammation
 Kidney Diseases Antibodies  >4 publications with RPLP0 and Kidney Diseases
 Lung Neoplasms Antibodies  >2 publications with RPLP0 and Lung Neoplasms
 Poisoning Antibodies  >2 publications with RPLP0 and Poisoning
 Liver Diseases Antibodies  >2 publications with RPLP0 and Liver Diseases
 Colonic Neoplasms Antibodies  >2 publications with RPLP0 and Colonic Neoplasms
 Nervous System Malformations Antibodies  >2 publications with RPLP0 and Nervous System Malformations

Organs/Tissues associated with anti-RPLP0 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with RPLP0 and Blood
 Brain Antibodies  >8 publications with RPLP0 and Brain
 Liver Antibodies  >7 publications with RPLP0 and Liver
 Lung Antibodies  >6 publications with RPLP0 and Lung
 Kidney Antibodies  >5 publications with RPLP0 and Kidney
 Intestine Antibodies  >5 publications with RPLP0 and Intestine
 Muscle Antibodies  >5 publications with RPLP0 and Muscle
 Skin Antibodies  >4 publications with RPLP0 and Skin
 Adipose Tissue Antibodies  >4 publications with RPLP0 and Adipose Tissue
 Connective Tissue Antibodies  >4 publications with RPLP0 and Connective Tissue
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