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P19 Ink4d/CDKN2D (CDKN2D) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755353

Cyclin-dependent kinase 4 inhibitor D is an enzyme that in humans is encoded by the CDKN2D gene. The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with… More details

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Product Overview

Online Datasheet in PDF
Product Name
p19 INK4d/CDKN2D, Polyclonal Antibody
Full Product Name
Anti-p19 INK4d/CDKN2D Antibody Picoband
Product Gene Name
anti-CDKN2D antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
17,700 Da
Chromosome Location
Chromosome: 19; NC_000019.9 (10677138..10679655, complement). Location: 19p13
OMIM
600927
3D Structure
ModBase 3D Structure for P55273
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human p19 INK4d/CDKN2D recombinant protein (Position: E31-H132).

Product Categories/Family for anti-CDKN2D antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CDKN2D antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-CDKN2D antibody
WB: 0.25-0.5 ug/ml, Human
FC/FACS: 1-3 ug/1x10^6 cells, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-CDKN2D antibody

Figure 1. Western blot analysis of P19 INK4d/CDKN2D using anti-P19 INK4d/CDKN2D antibody (MBS1755353).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human MOLT4 whole cell lysates,
Lane 2: human HEL whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P19 INK4d/CDKN2D antigen affinity purified polyclonal antibody (#MBS1755353) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P19 INK4d/CDKN2D at approximately 18 kDa. The expected band size for P19 INK4d/CDKN2D is at 18 kDa.
Rabbit Anti-CDKN2D Polyclonal Antibody – Cat# MBS1755353

Flow Cytometry (FC/FACS) of anti-CDKN2D antibody

Figure 2. Flow Cytometry analysis of U87 cells using anti-P19 INK4d/CDKN2D antibody (MBS1755353).

Overlay histogram showing U87 cells stained with MBS1755353 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P19 INK4d/CDKN2D Antibody (MBS1755353, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-CDKN2D Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1755353

Other Notes

Small volumes of anti-CDKN2D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDKN2D purchase

MBS1755353 is an antibody targeting CDKN2D. Also known as Anti-p19 INK4d/CDKN2D Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDKN2D. It may not necessarily be applicable to this product.

NCBI GI #
4502753
NCBI GeneID
1032
NCBI Accession #
NP_001791.1
UniProt Primary Accession #
P55273
UniProt Secondary Accession #
Q13102; Q6FGE9
UniProt Related Accession #
P55273
GenBank Nucleotide #
NM_001800.3
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor D
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
NCBI Official Symbol
CDKN2D
NCBI Official Synonym Symbols
p19; INK4D; p19-INK4D
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor D
UniProt Synonym Protein Names
p19-INK4d
Protein Family
Cyclin-dependent kinase 4 inhibitor
UniProt Gene Name
CDKN2D
UniProt Entry Name
CDN2D_HUMAN
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor D; CDK inhibitor p19INK4d; inhibitor of cyclin-dependent kinase 4d; cyclin-dependent kinase 4 inhibitor D p19; cell cycle inhibitor, Nur77 associating protein

NCBI Summary for CDKN2D

The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDKN2D

CDKN2D: Interacts strongly with CDK4 and CDK6 and inhibits them. Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein type: Tumor suppressor; Inhibitor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding

Biological Process: response to retinoic acid; negative regulation of caspase activity; negative regulation of cell proliferation; DNA synthesis during DNA repair; response to vitamin D; sensory perception of sound; autophagic cell death; negative regulation of phosphorylation; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; negative regulation of cell growth; cell cycle arrest; G1/S transition of mitotic cell cycle; response to UV
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Research Articles on CDKN2D

1. Up-regulation of CDKN2D during in-vitro passages of human amniotic fluid-derived mesenchymal stromal cells may indicate the begging of early senescence process in a p53-independent mechanism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDKN2D antibody

 Reference Product  PubMed Publications
 CDK4 antibody  >46 publications with CDKN2D and CDK4
 CDK6 antibody  >31 publications with CDKN2D and CDK6

Pathways associated with anti-CDKN2D antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin D Associated Events In G1 Pathway antibodies  Cyclin D Associated Events In G1 Pathway Diagram
 G1 Phase Pathway antibodies  G1 Phase Pathway Diagram
 G1 To S Cell Cycle Control Pathway antibodies  G1 To S Cell Cycle Control Pathway Diagram
 Mitotic G1-G1/S Phases Pathway antibodies  Mitotic G1-G1/S Phases Pathway Diagram

Diseases associated with anti-CDKN2D antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >59 publications with CDKN2D and Neoplasms
 Carcinoma Antibodies  >12 publications with CDKN2D and Carcinoma
 Nervous System Diseases Antibodies  >7 publications with CDKN2D and Nervous System Diseases
 Lymphoma Antibodies  >7 publications with CDKN2D and Lymphoma
 Ovarian Neoplasms Antibodies  >5 publications with CDKN2D and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >5 publications with CDKN2D and Ovarian Diseases
 Neoplasms, Experimental Antibodies  >4 publications with CDKN2D and Neoplasms, Experimental
 Lung Diseases Antibodies  >4 publications with CDKN2D and Lung Diseases
 Carcinoma, Squamous Cell Antibodies  >4 publications with CDKN2D and Carcinoma, Squamous Cell
 Kidney Diseases Antibodies  >3 publications with CDKN2D and Kidney Diseases

Organs/Tissues associated with anti-CDKN2D antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14 publications with CDKN2D and Blood
 Brain Antibodies  >12 publications with CDKN2D and Brain
 Bone Antibodies  >11 publications with CDKN2D and Bone
 Testis Antibodies  >8 publications with CDKN2D and Testis
 Lung Antibodies  >6 publications with CDKN2D and Lung
 Liver Antibodies  >4 publications with CDKN2D and Liver
 Embryonic Tissue Antibodies  >3 publications with CDKN2D and Embryonic Tissue
 Prostate Antibodies  >3 publications with CDKN2D and Prostate
 Skin Antibodies  >2 publications with CDKN2D and Skin
 Lymph Node Antibodies  >2 publications with CDKN2D and Lymph Node
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