• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GIT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755358

ARF GTPase-activating protein GIT2 is an enzyme that in humans is encoded by the GIT2 gene. This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF)… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GIT2, Polyclonal Antibody
Full Product Name
Anti-GIT2 Antibody Picoband
Product Gene Name
anti-GIT2 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
84,543 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (110367607..110434194, complement). Location: 12q24.1
OMIM
608564
3D Structure
ModBase 3D Structure for Q14161
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human GIT2 recombinant protein (Position: D400-S556).

Product Categories/Family for anti-GIT2 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-GIT2 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-GIT2 antibody
WB: 0.1-0.25 ug/ml, Human, Mouse
FC/FACS: 1-3 ug/1x10^6 cells, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-GIT2 antibody

Figure 1. Western blot analysis of GIT2 using anti-GIT2 antibody (MBS1755358).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: mouse lung tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIT2 antigen affinity purified polyclonal antibody (#MBS1755358) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GIT2 at approximately 85 kDa. The expected band size for GIT2 is at 85 kDa.
Rabbit Anti-GIT2 Polyclonal Antibody – Cat# MBS1755358

Flow Cytometry (FC/FACS) of anti-GIT2 antibody

Figure 2. Flow Cytometry analysis of PC-3 cells using anti-GIT2 antibody (MBS1755358).

Overlay histogram showing PC-3 cells stained with MBS1755358 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GIT2 Antibody (MBS1755358, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-GIT2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1755358

Other Notes

Small volumes of anti-GIT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GIT2 purchase

MBS1755358 is an antibody targeting GIT2. Also known as Anti-GIT2 Antibody Picoband. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GIT2. It may not necessarily be applicable to this product.

NCBI GI #
206725420
NCBI GeneID
9815
NCBI Accession #
NP_001128685.1
UniProt Primary Accession #
Q14161
UniProt Secondary Accession #
Q86U59; Q96CI2; Q9BV91; Q9Y5V2
UniProt Related Accession #
Q14161
GenBank Nucleotide #
NM_001135213.1
NCBI Official Full Name
ARF GTPase-activating protein GIT2 isoform 6
NCBI Official Synonym Full Names
G protein-coupled receptor kinase interacting ArfGAP 2
NCBI Official Symbol
GIT2
NCBI Official Synonym Symbols
CAT2; CAT-2
UniProt Protein Name
ARF GTPase-activating protein GIT2
UniProt Synonym Protein Names
Cool-interacting tyrosine-phosphorylated protein 2; CAT-2; CAT2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2
Protein Family
ARF GTPase-activating protein
UniProt Gene Name
GIT2
UniProt Synonym Gene Names
KIAA0148; ARF GAP GIT2; CAT-2; CAT2
UniProt Entry Name
GIT2_HUMAN
NCBI Protein Information
ARF GTPase-activating protein GIT2; ARF GAP GIT2; GRK-interacting protein 2; G protein-coupled receptor kinase-interactor 2; cool-associated, tyrosine phosphorylated protein 2; cool-interacting tyrosine-phosphorylated protein 2

NCBI Summary for GIT2

This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. [provided by RefSeq, Sep 2008]
Show more

UniProt Comments for GIT2

GIT2: possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Regulates Golgi and actin cytoskeletal organization. Important in integrin-mediated cell adhesion. Ten alternatively spliced isoforms have been described and additional isoforms seem to exist. Contains 1 Arf-GAP domain and 3 ANK repeats.

Protein type: GAPs, ARF; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; focal adhesion

Molecular Function: protein binding; zinc ion binding

Biological Process: behavioral response to pain; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
Show more

Research Articles on GIT2

1. The expression level of lymphocyte GRK might show the severity of CHF, and ACEI treatment could reduce the level of GRK in CHF patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GIT2 antibody

 Reference Product  PubMed Publications
 GIT1 antibody  >27 publications with GIT2 and GIT1
 PXN antibody  >5 publications with GIT2 and PXN
 PAK1 antibody  >3 publications with GIT2 and PAK1
 PAK3 antibody  >2 publications with GIT2 and PAK3
 ADRBK1 antibody  >1 publications with GIT2 and ADRBK1

Pathways associated with anti-GIT2 antibody

 Products by Pathway  Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Integrin-mediated Cell Adhesion Pathway antibodies  Integrin-mediated Cell Adhesion Pathway Diagram
 Signaling Events Mediated By Focal Adhesion Kinase Pathway antibodies  Signaling Events Mediated By Focal Adhesion Kinase Pathway Diagram

Diseases associated with anti-GIT2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with GIT2 and Neoplasms
 Pancreatic Neoplasms Antibodies  >1 publications with GIT2 and Pancreatic Neoplasms
 Learning Disorders Antibodies  >1 publications with GIT2 and Learning Disorders
 Inflammation Antibodies  >1 publications with GIT2 and Inflammation

Organs/Tissues associated with anti-GIT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with GIT2 and Blood
 Brain Antibodies  >7 publications with GIT2 and Brain
 Vascular Antibodies  >6 publications with GIT2 and Vascular
 Lung Antibodies  >5 publications with GIT2 and Lung
 Muscle Antibodies  >2 publications with GIT2 and Muscle
 Bone Antibodies  >2 publications with GIT2 and Bone
 Connective Tissue Antibodies  >1 publications with GIT2 and Connective Tissue
 Kidney Antibodies  >1 publications with GIT2 and Kidney
 Ovary Antibodies  >1 publications with GIT2 and Ovary
 Thymus Antibodies  >1 publications with GIT2 and Thymus
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000