Rabbit Anti-P2RX4 Polyclonal Antibody – Cat# MBS1755394

Figure 1. Western blot analysis of P2X4/P2RX4 using anti-P2X4/P2RX4 antibody (MBS1755394).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X4/P2RX4 antigen affinity purified polyclonal antibody (#MBS1755394) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P2X4/P2RX4 at approximately 70 kDa. The expected band size for P2X4/P2RX4 is at 43 kDa.

P2X4/P2RX4 (P2RX4) Antibody – Rabbit Polyclonal

Name: P2RX4 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1755394
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755394

Purinoceptor P2X4, also called P2X4R is a protein that in humans is encoded by the P2RX4 gene. This gene belongs to the family of purinoceptors for ATP. P2RX4 was mapped to 12q24.32 by fluorescence in situ hybridization. P2RX4 is a receptor for ATP that acts… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

P2X4/P2RX4, Polyclonal Antibody

Full Product Name

Anti-P2X4/P2RX4 Antibody Picoband

Product Gene Name

anti-P2RX4 antibody

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

Molecular Weight

40,481 Da

OMIM

600846

3D Structure

ModBase 3D Structure for Q99571

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Immunogen

E Coli-derived human P2X4/P2RX4 recombinant protein (Position: Q111-E249).

Product Categories/Family for anti-P2RX4 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-P2RX4 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-P2RX4 antibody

WB: 0.25-0.5 ug/ml, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Other Notes

Small volumes of anti-P2RX4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P2RX4 purchase

MBS1755394 is an antibody targeting P2RX4. Also known as Anti-P2X4/P2RX4 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for P2RX4. It may not necessarily be applicable to this product.

NCBI GI #

116242696

NCBI GeneID

5025

NCBI Accession #

Q99571.2

UniProt Primary Accession #

Q99571

UniProt Secondary Accession #

O00450; O14722; Q5U089; Q5U090; Q8N4N1; Q9UBG9; E7EPF7; F6RU17

UniProt Related Accession #

Q99571

NCBI Official Full Name

P2X purinoceptor 4

NCBI Official Synonym Full Names

purinergic receptor P2X, ligand-gated ion channel, 4

NCBI Official Symbol

P2RX4

NCBI Official Synonym Symbols

P2X4; P2X4R

UniProt Protein Name

P2X purinoceptor 4

UniProt Synonym Protein Names

ATP receptor; Purinergic receptor

Protein Family

P2X purinoceptor

UniProt Gene Name

P2RX4

UniProt Synonym Gene Names

P2X4

UniProt Entry Name

P2RX4_HUMAN

NCBI Protein Information

P2X purinoceptor 4; ATP receptor; purinoceptor P2X4; P2X receptor, subunit 4; purinergic receptor P2X4; ATP-gated cation channel protein

NCBI Summary for P2RX4

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]

UniProt Comments for P2RX4

P2X4: Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin. Belongs to the P2X receptor family.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.32

Cellular Component: membrane; cell soma; perinuclear region of cytoplasm; apical part of cell; lysosomal membrane; integral to plasma membrane; postsynaptic density; dendritic spine; plasma membrane; terminal button; integral to nuclear inner membrane; cell junction

Molecular Function: protein homodimerization activity; copper ion binding; zinc ion binding; cadherin binding; drug binding; ATP binding; purinergic nucleotide receptor activity; ATP-gated cation channel activity; receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; generation of action potential; positive regulation of calcium-mediated signaling; regulation of sodium ion transport; sensory perception of pain; signal transduction; vasodilation; response to ATP; endothelial cell activation; membrane depolarization; transport; calcium ion transport; regulation of blood pressure; positive regulation of prostaglandin secretion; tissue homeostasis; regulation of excitatory postsynaptic membrane potential; positive regulation of calcium ion transport; nitric oxide biosynthetic process; protein homooligomerization

Research Articles on P2RX4

1. the lysosome-localized P2X4 may play specific roles in membrane trafficking of acidic organelles in mammalian cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-P2RX4 antibody

Reference Product	PubMed Publications
P2RY2 antibody	>8 publications with P2RX4 and P2RY2
ACCN2 antibody	>1 publications with P2RX4 and ACCN2
PLA2G1B antibody	>1 publications with P2RX4 and PLA2G1B
GATA2 antibody	>1 publications with P2RX4 and GATA2

Pathways associated with anti-P2RX4 antibody

Products by Pathway	Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with anti-P2RX4 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>38 publications with P2RX4 and Nervous System Diseases
Inflammation Antibodies	>16 publications with P2RX4 and Inflammation
Disease Models, Animal Antibodies	>14 publications with P2RX4 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>12 publications with P2RX4 and Cardiovascular Diseases
Hypertension Antibodies	>7 publications with P2RX4 and Hypertension
Necrosis Antibodies	>5 publications with P2RX4 and Necrosis
Immune System Diseases Antibodies	>4 publications with P2RX4 and Immune System Diseases
Hypertrophy Antibodies	>3 publications with P2RX4 and Hypertrophy
Liver Neoplasms Antibodies	>3 publications with P2RX4 and Liver Neoplasms
Liver Diseases Antibodies	>3 publications with P2RX4 and Liver Diseases

Organs/Tissues associated with anti-P2RX4 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>65 publications with P2RX4 and Brain
Kidney Antibodies	>21 publications with P2RX4 and Kidney
Heart Antibodies	>21 publications with P2RX4 and Heart
Muscle Antibodies	>20 publications with P2RX4 and Muscle
Blood Antibodies	>18 publications with P2RX4 and Blood
Bone Antibodies	>11 publications with P2RX4 and Bone
Ganglia Antibodies	>8 publications with P2RX4 and Ganglia
Mouth Antibodies	>8 publications with P2RX4 and Mouth
Lung Antibodies	>6 publications with P2RX4 and Lung
Eye Antibodies	>6 publications with P2RX4 and Eye