Figure 1. Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (MBS1755640).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human CACO-2 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (#MBS1755640) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.
Figure 2. Western blot analysis of NDUFS8 using anti-NDUFS8 antibody (MBS1755640).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse skeletal muscle tissue lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS8 antigen affinity purified polyclonal antibody (#MBS1755640) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDUFS8 at approximately 23 kDa. The expected band size for NDUFS8 is at 23 kDa.
Figure 3. IHC analysis of NDUFS8 using anti-NDUFS8 antibody (MBS1755640).
NDUFS8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NDUFS8 Antibody (MBS1755640) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Figure 4. IHC analysis of NDUFS8 using anti-NDUFS8 antibody (MBS1755640).
NDUFS8 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-NDUFS8 Antibody (MBS1755640) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Figure 5. Flow Cytometry analysis of HeLa cells using anti-NDUFS8 antibody (MBS1755640).
Overlay histogram showing HeLa cells stained with MBS1755640 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (MBS1755640, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 6. Flow Cytometry analysis of 293T cells using anti-NDUFS8 antibody (MBS1755640).
Overlay histogram showing 293T cells stained with MBS1755640 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (MBS1755640, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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