• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

POLA2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755651

DNA polymerase alpha subunit 2 is an enzyme that in humans is encoded by the POLA2 gene. POLA2 may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLA2, Polyclonal Antibody
Full Product Name
Anti-POLA2 Antibody Picoband
Product Gene Name
anti-POLA2 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
Molecular Weight
598
3D Structure
ModBase 3D Structure for Q14181
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human POLA2 recombinant protein (Position: N267-P579).

Product Categories/Family for anti-POLA2 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-POLA2 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes for anti-POLA2 antibody
WB: 0.25-0.5 ug/ml, Human
FC/FACS: 1-3 ug/1x10^6 cells, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Western Blot (WB) of anti-POLA2 antibody

Figure 1. Western blot analysis of POLA2 using anti-POLA2 antibody (MBS1755651).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human COLO320 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLA2 antigen affinity purified polyclonal antibody (#MBS1755651) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POLA2 at approximately 70 kDa. The expected band size for POLA2 is at 66 kDa.
Rabbit Anti-POLA2 Polyclonal Antibody – Cat# MBS1755651

Flow Cytometry (FC/FACS) of anti-POLA2 antibody

Figure 2. Flow Cytometry analysis of U251 cells using anti-POLA2 antibody (MBS1755651).

Overlay histogram showing U251 cells stained with MBS1755651 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLA2 Antibody (MBS1755651, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-POLA2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1755651

Other Notes

Small volumes of anti-POLA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLA2 purchase

MBS1755651 is an antibody targeting POLA2. Also known as Anti-POLA2 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLA2. It may not necessarily be applicable to this product.

NCBI GI #
90110415
NCBI GeneID
23649
NCBI Accession #
Q14181.2
UniProt Primary Accession #
Q14181
UniProt Secondary Accession #
Q9BPV3; B4DNB4
UniProt Related Accession #
Q14181
NCBI Official Full Name
DNA polymerase alpha subunit B
NCBI Official Synonym Full Names
polymerase (DNA directed), alpha 2, accessory subunit
NCBI Official Symbol
POLA2
UniProt Protein Name
DNA polymerase alpha subunit B
UniProt Synonym Protein Names
DNA polymerase alpha 70 kDa subunit
Protein Family
DNA polymerase
UniProt Gene Name
POLA2
UniProt Entry Name
DPOA2_HUMAN
NCBI Protein Information
DNA polymerase alpha subunit B; DNA polymerase alpha 70 kDa subunit; polymerase (DNA-directed), alpha (70kD); polymerase (DNA directed), alpha 2 (70kD subunit)

UniProt Comments for POLA2

POLA2: a DNA polymerase that may play an essential role at the early stage of chromosomal DNA replication by coupling the polymerase alpha/primase complex to the cellular replication machinery. DNA polymerase alpha-primase is a four subunit enzyme (subunits A, B, C and D), which is assembled throughout the cell cycle. The largest subunit (subunit A) has DNA polymerase activity, the two smallest subunits (subunits C and D) have DNA primase activity. Subunit B binds to subunit A. Phosphorylated in a cell cycle-dependent manner, in G2/M phase.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; DNA replication

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: nucleoplasm; alpha DNA polymerase:primase complex; cytoplasm

Molecular Function: DNA binding; protein heterodimerization activity; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; DNA replication initiation; protein import into nucleus, translocation; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
Show more

Research Articles on POLA2

1. Findings indicate that tethering of primase to the replisome by DNA polymerase alpha (pol alpha) is critical for the normal action of DNA replication forks in eukaryotic cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLA2 antibody

 Reference Product  PubMed Publications
 POLD1 antibody  >2 publications with POLA2 and POLD1
 POLA1 antibody  >2 publications with POLA2 and POLA1

Pathways associated with anti-POLA2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram
 DNA Replication Pre-Initiation Pathway antibodies  DNA Replication Pre-Initiation Pathway Diagram
 DNA Polymerase Alpha / Primase Complex Pathway antibodies  DNA Polymerase Alpha / Primase Complex Pathway Diagram
 DNA Polymerase Alpha / Primase Complex Pathway antibodies  DNA Polymerase Alpha / Primase Complex Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Organs/Tissues associated with anti-POLA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1 publications with POLA2 and Lung
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000