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TRMT5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755788

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using… More details

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Product Overview

Online Datasheet in PDF
Product Name
TRMT5, Polyclonal Antibody
Full Product Name
Anti-TRMT5 Antibody Picoband
Product Gene Name
anti-TRMT5 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
58,246 Da
OMIM
611023
3D Structure
ModBase 3D Structure for Q32P41
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Immunogen
E Coli-derived human TRMT5 recombinant protein (Position: R291-E501).

Product Categories/Family for anti-TRMT5 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TRMT5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-TRMT5 antibody
WB: 0.25-0.5 ug/ml, Human
ICC/IF: 5 ug/ml, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (#MBS176453) for ICC.

Western Blot (WB) of anti-TRMT5 antibody

Figure 1. Western blot analysis of TRMT5 using anti-TRMT5 antibody (MBS1755788).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human MCF-7 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT5 antigen affinity purified polyclonal antibody (#MBS1755788) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRMT5 at approximately 50 kDa. The expected band size for TRMT5 is at 58 kDa.
Rabbit Anti-TRMT5 Polyclonal Antibody – Cat# MBS1755788

Immunofluorescence (IF) of anti-TRMT5 antibody

Figure 2. IF analysis of TRMT5 using anti-TRMT5 antibody (MBS1755788) and anti-Tubulin Alpha antibody (M03989-3).

TRMT5 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TRMT5 Antibody (MBS1755788) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (MBS177407) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-TRMT5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1755788

Other Notes

Small volumes of anti-TRMT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRMT5 purchase

MBS1755788 is an antibody targeting TRMT5. Also known as Anti-TRMT5 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRMT5. It may not necessarily be applicable to this product.

NCBI GI #
612339294
NCBI GeneID
57570
NCBI Accession #
NP_065861.3
UniProt Primary Accession #
Q32P41
UniProt Secondary Accession #
Q9P2F4; B2RN19
UniProt Related Accession #
Q32P41
GenBank Nucleotide #
NM_020810.3
NCBI Official Full Name
tRNA (guanine(37)-N1)-methyltransferase
NCBI Official Synonym Full Names
tRNA methyltransferase 5
NCBI Official Symbol
TRMT5
NCBI Official Synonym Symbols
TRM5; KIAA1393
UniProt Protein Name
tRNA (guanine(37)-N1)-methyltransferase
UniProt Synonym Protein Names
M1G-methyltransferase
UniProt Gene Name
TRMT5
UniProt Entry Name
TRM5_HUMAN
NCBI Protein Information
tRNA (guanine(37)-N1)-methyltransferase

NCBI Summary for TRMT5

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM, Mar 2008]
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UniProt Comments for TRMT5

TRMT5: Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Belongs to the TRM5 / TYW2 family.

Protein type: EC 2.1.1.228; Methyltransferase

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: mitochondrial matrix; nucleus

Biological Process: tRNA methylation

Disease: Combined Oxidative Phosphorylation Deficiency 26
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Research Articles on TRMT5

1. Conservation of structure and mechanism by Trm5 enzymes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-TRMT5 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with TRMT5 and Heart
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