Mouse Anti-XIAP Monoclonal Antibody – Cat# MBS1756547

Figure 1. Western blot analysis of XIAP using anti-XIAP antibody (MBS1756547).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse testis tissue lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XIAP antigen affinity purified monoclonal antibody (#MBS1756547) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for XIAP at approximately 54 kDa. The expected band size for XIAP is at 57 kDa.

XIAP Antibody – Mouse Monoclonal

Name: XIAP Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1756547
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1756547

XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death. It is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed,… More details

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Testing Data View PDF

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

XIAP, Monoclonal Antibody

Full Product Name

Anti-XIAP Antibody Picoband (monoclonal, 3G2G1)

Product Gene Name

anti-XIAP antibody

Clonality

Monoclonal

Isotype

Mouse IgG1

Clone Number

3G2G1

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Form/Format

Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

Molecular Weight

56,685 Da

Chromosome Location

Chromosome: X; NC_000023.10 (122993662..123047829). Location: Xq25

OMIM

300079

3D Structure

ModBase 3D Structure for P98170

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Immunogen

E Coli-derived human XIAP recombinant protein (Position: A15-V244). Human XIAP shares 89.4% and 90.7% amino acid (aa) sequence identity with mouse and rat XIAP, respectively.

Product Categories/Family for anti-XIAP antibody

Primary Antibodies, Mouse Monoclonal Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-XIAP antibody

Western Blot (WB)

Application Notes for anti-XIAP antibody

WB: 0.25-0.5 ug/ml, Human, Mouse, Rat
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Mouse IgG (#MBS176445) for Western blot.

Other Notes

Small volumes of anti-XIAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for XIAP purchase

MBS1756547 is an antibody targeting XIAP. Also known as Anti-XIAP Antibody Picoband (monoclonal, 3G2G1). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for XIAP. It may not necessarily be applicable to this product.

NCBI GI #

32528299

NCBI GeneID

331

NCBI Accession #

NP_001158.2

UniProt Primary Accession #

P98170

UniProt Secondary Accession #

Q9NQ14; D3DTF2

UniProt Related Accession #

P98170

GenBank Nucleotide #

NM_001167.3

NCBI Official Full Name

E3 ubiquitin-protein ligase XIAP

NCBI Official Synonym Full Names

X-linked inhibitor of apoptosis

NCBI Official Symbol

XIAP

NCBI Official Synonym Symbols

API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3

UniProt Protein Name

E3 ubiquitin-protein ligase XIAP

UniProt Synonym Protein Names

Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

XIAP

UniProt Synonym Gene Names

API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3

UniProt Entry Name

XIAP_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase XIAP; X-linked IAP; IAP-like protein; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing protein 4

NCBI Summary for XIAP

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

UniProt Comments for XIAP

XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Apoptosis

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; regulation of inflammatory response; regulation of BMP signaling pathway; protein ubiquitination; negative regulation of caspase activity; response to DNA damage stimulus; copper ion homeostasis; regulation of innate immune response; negative regulation of apoptosis; regulation of cell proliferation

Disease: Lymphoproliferative Syndrome, X-linked, 1; Lymphoproliferative Syndrome, X-linked, 2

Research Articles on XIAP

1. Downregulation of uPAR and cathepsin B quenched TGF-beta1-driven invasion and survival of meningioma cells in a XIAP-dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-XIAP antibody

Reference Product	PubMed Publications
CASP3 antibody	>752 publications with XIAP and CASP3
CASP9 antibody	>370 publications with XIAP and CASP9
DIABLO antibody	>277 publications with XIAP and DIABLO
BIRC5 antibody	>167 publications with XIAP and BIRC5
HTRA2 antibody	>73 publications with XIAP and HTRA2
CASP7 antibody	>71 publications with XIAP and CASP7
AKT1 antibody	>38 publications with XIAP and AKT1
TAB1 antibody	>10 publications with XIAP and TAB1
COMMD1 antibody	>6 publications with XIAP and COMMD1
MAGED1 antibody	>5 publications with XIAP and MAGED1

Pathways associated with anti-XIAP antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Factor-mediated Response Pathway antibodies	Apoptotic Factor-mediated Response Pathway Diagram
BMP Receptor Signaling Pathway antibodies	BMP Receptor Signaling Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram

Diseases associated with anti-XIAP antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>1129 publications with XIAP and Neoplasms
Necrosis Antibodies	>389 publications with XIAP and Necrosis
Leukemia Antibodies	>315 publications with XIAP and Leukemia
Lymphoproliferative Disorders Antibodies	>197 publications with XIAP and Lymphoproliferative Disorders
Adenocarcinoma Antibodies	>145 publications with XIAP and Adenocarcinoma
Nervous System Diseases Antibodies	>142 publications with XIAP and Nervous System Diseases
Gastrointestinal Diseases Antibodies	>117 publications with XIAP and Gastrointestinal Diseases
Drug Toxicity Antibodies	>98 publications with XIAP and Drug Toxicity
Brain Diseases Antibodies	>93 publications with XIAP and Brain Diseases
Neoplasms, Experimental Antibodies	>91 publications with XIAP and Neoplasms, Experimental

Organs/Tissues associated with anti-XIAP antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>142 publications with XIAP and Brain
Muscle Antibodies	>59 publications with XIAP and Muscle
Kidney Antibodies	>45 publications with XIAP and Kidney
Skin Antibodies	>33 publications with XIAP and Skin
Embryonic Tissue Antibodies	>21 publications with XIAP and Embryonic Tissue
Placenta Antibodies	>17 publications with XIAP and Placenta
Uterus Antibodies	>13 publications with XIAP and Uterus
Thymus Antibodies	>9 publications with XIAP and Thymus
Mammary Gland Antibodies	>8 publications with XIAP and Mammary Gland
Cervix Antibodies	>2 publications with XIAP and Cervix