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CD95 (FAS) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175670

Description: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P, IHC-F in Mouse, Rat.Background: FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD95 (FAS), Polyclonal Antibody
Full Product Name
Anti-CD95 antibody
Product Gene Name
anti-FAS antibody
Product Synonym Names
Tumor necrosis factor receptor superfamily member 6; Fas(TNF receptor superfamily, member 6); ALPS 1A antibody; ALPS1A antibody; APO 1 antibody; Apo 1 antigen antibody; APO 1 cell surface antigen antibody; Apo-1 antigen antibody; APO1 antibody; Apo1 antigen antibody; APO1 cell surface antigen antibody; Apoptosis antigen 1 antibody; Apoptosis mediating surface antigen FAS antibody; Apoptosis-mediating surface antigen FAS antibody; APT 1 antibody; APT1 antibody; CD 95 antibody; CD 95 antigen antibody; CD95 antibody; CD95 antigen antibody; Delta Fas antibody; Delta Fas/APO 1/CD95 antibody; Delta Fas/APO1/CD95 antibody; Fas(TNF receptor superfamily, member 6) antibody; FAS 1 antibody; FAS 827dupA antibody; Fas AMA antibody; FAS antibody; FAS Antigen antibody; Fas cell surface death receptor antibody; FAS1 antibody; FASLG receptor antibody; FASTM antibody; sFAS antibody; Surface antigen APO1 antibody; TNF receptor superfamily, member 6 antibody; TNFRSF 6 antibody; TNFRSF6 antibody; TNR6_HUMAN antibody; Tumor necrosis factor receptor superfamily member 6 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for Q63199
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat CD95 (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FAS antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
Application Notes for anti-FAS antibody
WB: Concentration: 0.1-0.5 ug/ml; Tested Species: Mouse, Rat
IHC (Paraffin-embedded Section): Concentration: 0.5-1 ug/ml; Tested Species: Mouse, Rat; Antigen Retrieval: By Heat
IHC (Frozen Section): Concentration 0.5-1 ug/ml; Tested Species: Mouse, Rat

Tested Species: In-house tested species with positive test results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-FAS antibody

Anti-Fas antibody, MBS175670, Western blotting
Lane 1: Recombinant Mouse FAS Protein 10ng
Lane 2: Recombinant Mouse FAS Protein 5ng
Lane 3: Recombinant Mouse FAS Protein 2.5ng
Rabbit Anti-FAS Polyclonal Antibody – Cat# MBS175670

Immunohistochemistry (IHC) of anti-FAS antibody

Anti-Fas antibody, MBS175670, IHC(P)
IHC(P): Rat Spleen Tissue Lysate
Rabbit Anti-FAS Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175670

Western Blot (WB) of anti-FAS antibody

Western blot analysis of FAS expression in rat liver extract (lane 1), rat spleen extract (lane 2), rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-FAS Polyclonal Antibody, Western Blot (WB) – Cat# MBS175670

Western Blot (WB) of anti-FAS antibody

Western blot analysis of FAS expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse brain extract (lane 3) mouse kidney extract (lane 4), mouse thymus extract (lane 5), mouse lung extract (lane 6), HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
Rabbit Anti-FAS Polyclonal Antibody, Western Blot (WB) – Cat# MBS175670

Other Notes

Small volumes of anti-FAS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAS purchase

MBS175670 is an antibody targeting FAS. Also known as Anti-CD95 antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FAS. It may not necessarily be applicable to this product.

NCBI GI #
6015131
NCBI GeneID
246097
NCBI Accession #
Q63199.1
UniProt Primary Accession #
Q63199
UniProt Related Accession #
Q63199
NCBI Official Full Name
Tumor necrosis factor receptor superfamily member 6
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
Fas
NCBI Official Synonym Symbols
Tnfrsf6
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6
UniProt Synonym Protein Names
Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen: CD95
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
Fas
UniProt Synonym Gene Names
Apt1; Tnfrsf6
UniProt Entry Name
TNR6_RAT
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6; CD95 antigen; Fas receptor; apo-1 antigen; FASLG receptor; Fas antigen (ATP1); apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); Tumor necrosis factor receptor superfamily, member 6

NCBI Summary for FAS

Tnfsf6/Fasl receptor [RGD, Feb 2006]

UniProt Comments for FAS

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both ().
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Research Articles on FAS

1. Kruppel-like factor 6 rendered rat Schwann cell more sensitive to apoptosis via upregulating FAS expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FAS antibody

 Reference Product  PubMed Publications
 Faslg antibody  >3567 publications with FAS and Faslg
 Casp8 antibody  >2914 publications with FAS and Casp8
 Casp3 antibody  >2913 publications with FAS and Casp3
 Fadd antibody  >1871 publications with FAS and Fadd
 Egfr antibody  >102 publications with FAS and Egfr
 Daxx antibody  >100 publications with FAS and Daxx
 Erbb2 antibody  >66 publications with FAS and Erbb2
 Il4 antibody  >18 publications with FAS and Il4
 Ifngr2 antibody  >2 publications with FAS and Ifngr2
 Ifngr1 antibody  >1 publications with FAS and Ifngr1

Pathways associated with anti-FAS antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram

Diseases associated with anti-FAS antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >4948 publications with FAS and Necrosis
 Autoimmune Diseases Antibodies  >2001 publications with FAS and Autoimmune Diseases
 Inflammation Antibodies  >1726 publications with FAS and Inflammation
 Nervous System Diseases Antibodies  >1533 publications with FAS and Nervous System Diseases
 Disease Models, Animal Antibodies  >1445 publications with FAS and Disease Models, Animal
 Liver Diseases Antibodies  >1404 publications with FAS and Liver Diseases
 Fatty Liver Antibodies  >960 publications with FAS and Fatty Liver
 Hematologic Diseases Antibodies  >663 publications with FAS and Hematologic Diseases
 Heart Diseases Antibodies  >535 publications with FAS and Heart Diseases
 Kidney Diseases Antibodies  >533 publications with FAS and Kidney Diseases

Organs/Tissues associated with anti-FAS antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >1190 publications with FAS and Lung
 Thymus Antibodies  >419 publications with FAS and Thymus
 Pancreas Antibodies  >370 publications with FAS and Pancreas
 Embryonic Tissue Antibodies  >313 publications with FAS and Embryonic Tissue
 Small Intestine Antibodies  >137 publications with FAS and Small Intestine
 Uncharacterized Tissue Antibodies  >4 publications with FAS and Uncharacterized Tissue
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