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Il-8 (IL8) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175692

Description: Rabbit IgG polyclonal antibody for Interleukin-8(IL8) detection. Tested with WB, IHC-P in Human.Background: Interleukin-8(IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IL-8 (IL8), Polyclonal Antibody
Full Product Name
Anti-IL-8 antibody
Product Gene Name
anti-IL8 antibody
Product Synonym Names
Interleukin-8(IL-8); interleukin 8; (Ala-IL-8)77 antibody; (Ser-IL-8)72 antibody; 3 10C antibody; 310C antibody; AMCF 1 antibody; AMCF1 antibody; b ENAP antibody; Bbeta thromboglobulin like protein antibody; Beta thromboglobulin like protein antibody; C-X-C motif chemokine 8 antibody; CXC chemokine ligand 8 antibody; CXCL 8 antibody; CXCL8 antibody; Emoctakin antibody; GCP 1 antibody; GCP-1 antibody; GCP/IL-8 protein I antibody; GCP/IL-8 protein II antibody; GCP/IL-8 protein III antibody; GCP/IL-8 protein IV antibody; GCP/IL-8 protein V antibody; GCP/IL-8 protein VI antibody; GCP1 antibody; Granulocyte chemotactic protein 1 antibody; IL 8 antibody; IL-8 antibody; IL-8(1-77) antibody; IL-8(9-77) antibody; IL8 antibody; IL8/NAP1 form I antibody; IL8/NAP1 form II antibody; IL8/NAP1 form III antibody; IL8/NAP1 form IV antibody; IL8/NAP1 form V antibody; IL8/NAP1 form VI antibody; IL8_HUMAN antibody; Inteleukin 8 antibody; Interleukin8 antibody; K 60 antibody; K60 antibody; LECT antibody; LUCT antibody; Lymphocyte derived neutrophil activating factor antibody; Lymphocyte-derived neutrophil-activating factor antibody; LYNAP antibody; MDNCF antibody; MDNCF-b antibody; MDNCF-c antibody; MONAP antibody; Monocyte derived neutrophil activating peptide antibody; Monocyte derived neutrophil activating protein antibody; Monocyte derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil-activating peptide antibody; NAF antibody; NAP 1 antibody; NAP-1 antibody; NAP1 antibody; Neutrophil activating factor antibody; Neutrophil activating peptide 1 antibody; Neutrophil activating protein 1 antibody; Neutrophil-activating factor antibody; Neutrophil-activating protein 1 antibody; Protein 3 10C antibody; Protein 3-10C antibody; SCYB 8 antibody; SCYB8 antibody; Small inducible cytokine subfamily B member 8 antibody; T cell chemotactic factor antibody; T-cell chemotactic factor antibody; TSG 1 antibody; TSG1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
11,338 Da
OMIM
146930
3D Structure
ModBase 3D Structure for P10145
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IL8 (77-95aa CLDPKENWVQRVVEKFLKR).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-IL8 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-IL8 antibody
Western Blot (WB):
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: -
Antigen Retrieval: -

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu
Predicted Species: -
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species prediceted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-IL8 antibody

Anti-IL-8 antibody, MBS175692, Western blotting
Lane 1: Recombinant Human IL-18 Protein 10ng
Lane 2: Recombinant Human IL-18 Protein 5ng
Lane 3: Recombinant Human IL-18 Protein 2.5ng
Rabbit Anti-IL8 Polyclonal Antibody – Cat# MBS175692

Immunohistochemistry (IHC) of anti-IL8 antibody

Anti-IL-8 antibody, MBS175692, IHC(P)
IHC(P): Human Appendicitis Tissue
Rabbit Anti-IL8 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS175692

Other Notes

Small volumes of anti-IL8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL8 purchase

MBS175692 is an antibody targeting IL8. Also known as Anti-IL-8 antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IL8. It may not necessarily be applicable to this product.

NCBI GI #
124359
NCBI GeneID
3576
NCBI Accession #
P10145.1
UniProt Primary Accession #
P10145
UniProt Secondary Accession #
Q6FGF6; Q6LAE6; Q96RG6; Q9C077; Q9UCE1; Q9UCR8; Q9UCR9; Q9UCS0; B2R4L8
UniProt Related Accession #
P10145
NCBI Official Full Name
Interleukin-8
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
UniProt Protein Name
Interleukin-8
UniProt Synonym Protein Names
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I
UniProt Gene Name
CXCL8
UniProt Synonym Gene Names
IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
UniProt Entry Name
IL8_HUMAN
NCBI Protein Information
interleukin-8; emoctakin; interleukin 8; T-cell chemotactic factor; neutrophil-activating peptide 1; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide

NCBI Summary for IL8

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
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UniProt Comments for IL8

IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding

Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; unfolded protein response; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; cell cycle arrest; cell motility; inflammatory response; embryonic gut development
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Research Articles on IL8

1. these data suggest that IL-8 plays an important role in breast cancer osteolysis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL8 antibody

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 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with anti-IL8 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >596 publications with IL8 and Inflammation
 Necrosis Antibodies  >314 publications with IL8 and Necrosis
 Pulmonary Disease, Chronic Obstructive Antibodies  >79 publications with IL8 and Pulmonary Disease, Chronic Obstructive
 Adenocarcinoma Antibodies  >32 publications with IL8 and Adenocarcinoma
 Breast Neoplasms Antibodies  >24 publications with IL8 and Breast Neoplasms
 Lung Neoplasms Antibodies  >21 publications with IL8 and Lung Neoplasms
 Kidney Diseases Antibodies  >20 publications with IL8 and Kidney Diseases
 Colorectal Neoplasms Antibodies  >15 publications with IL8 and Colorectal Neoplasms
 Pulmonary Fibrosis Antibodies  >15 publications with IL8 and Pulmonary Fibrosis
 Fever Antibodies  >14 publications with IL8 and Fever

Organs/Tissues associated with anti-IL8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >731 publications with IL8 and Blood
 Lung Antibodies  >230 publications with IL8 and Lung
 Vascular Antibodies  >196 publications with IL8 and Vascular
 Skin Antibodies  >122 publications with IL8 and Skin
 Bone Antibodies  >68 publications with IL8 and Bone
 Brain Antibodies  >57 publications with IL8 and Brain
 Muscle Antibodies  >50 publications with IL8 and Muscle
 Heart Antibodies  >50 publications with IL8 and Heart
 Intestine Antibodies  >49 publications with IL8 and Intestine
 Bone Marrow Antibodies  >47 publications with IL8 and Bone Marrow
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