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MIF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175770

Description: Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB, ELISA in Human.Background: Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MIF, Polyclonal Antibody
Full Product Name
Anti-human MIF antibody
Product Gene Name
anti-MIF antibody
Product Synonym Names
Macrophage migration inhibitory factor; macrophage migration inhibitory factor(glycosylation-inhibiting factor); acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody; GIF antibody; GLIF antibody; Glycosylation inhibiting factor antibody; Glycosylation-inhibiting factor antibody; L-dopachrome isomerase antibody; L-dopachrome tautomerase antibody; Macrophage migration inhibitory factor antibody; MIF antibody; MIF protein antibody; MMIF antibody; Phenylpyruvate tautomerase antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
12,476 Da
OMIM
gene 604302
3D Structure
ModBase 3D Structure for P14174
Immunogen
E Coli-derived human MIF recombinant protein (Position: M1-A115).
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MIF antibody
Western Blot (WB), ELISA (EIA)

Western Blot (WB) of anti-MIF antibody

Figure. Western blot analysis of MIF using anti- MIF antibody (MBS175770).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane : Recombinant Human MIF Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MIF antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIF at approximately 12KD. The expected band size for MIF is at 12KD.
Rabbit Anti-MIF Polyclonal Antibody – Cat# MBS175770

Other Notes

Small volumes of anti-MIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIF purchase

MBS175770 is an antibody targeting MIF. Also known as Anti-human MIF antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.

NCBI GI #
1170955
NCBI GeneID
4282
NCBI Accession #
P14174.4
UniProt Primary Accession #
P14174
UniProt Secondary Accession #
Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3
UniProt Related Accession #
P14174
NCBI Official Full Name
Macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Official Symbol
MIF
NCBI Official Synonym Symbols
GIF; GLIF; MMIF
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Synonym Protein Names
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
Protein Family
Macrophage migration inhibitory factor
UniProt Gene Name
MIF
UniProt Synonym Gene Names
GLIF; MMIF; MIF; GIF
UniProt Entry Name
MIF_HUMAN
NCBI Protein Information
macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase

NCBI Summary for MIF

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
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UniProt Comments for MIF

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - phenylalanine; EC 5.3.2.1; Amino Acid Metabolism - tyrosine; Apoptosis; EC 5.3.3.12; Cytokine; Cell development/differentiation; Isomerase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle

Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity

Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; cell proliferation; positive regulation of MAP kinase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; positive regulation of fibroblast proliferation; negative regulation of mature B cell apoptosis; cell surface receptor linked signal transduction; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; positive regulation of cytokine secretion; negative regulation of apoptosis

Disease: Rheumatoid Arthritis, Systemic Juvenile
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Research Articles on MIF

1. Increased expression of MIF and DJ-1 induced cell invasion and metastasis of nasopharyngeal carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MIF antibody

 Reference Product  PubMed Publications
 CD74 antibody  >138 publications with MIF and CD74
 COPS5 antibody  >17 publications with MIF and COPS5
 MMP9 antibody  >13 publications with MIF and MMP9
 TAT antibody  >3 publications with MIF and TAT
 UBC antibody  >2 publications with MIF and UBC
 NME1 antibody  >1 publications with MIF and NME1

Pathways associated with anti-MIF antibody

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram

Diseases associated with anti-MIF antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >684 publications with MIF and Inflammation
 Necrosis Antibodies  >319 publications with MIF and Necrosis
 Disease Models, Animal Antibodies  >227 publications with MIF and Disease Models, Animal
 Lung Diseases Antibodies  >211 publications with MIF and Lung Diseases
 Carcinoma Antibodies  >158 publications with MIF and Carcinoma
 Sepsis Antibodies  >137 publications with MIF and Sepsis
 Arthritis, Rheumatoid Antibodies  >112 publications with MIF and Arthritis, Rheumatoid
 Heart Diseases Antibodies  >103 publications with MIF and Heart Diseases
 Atherosclerosis Antibodies  >91 publications with MIF and Atherosclerosis
 Pneumonia Antibodies  >83 publications with MIF and Pneumonia

Organs/Tissues associated with anti-MIF antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1509 publications with MIF and Blood
 Brain Antibodies  >403 publications with MIF and Brain
 Vascular Antibodies  >236 publications with MIF and Vascular
 Skin Antibodies  >217 publications with MIF and Skin
 Lung Antibodies  >211 publications with MIF and Lung
 Muscle Antibodies  >198 publications with MIF and Muscle
 Spleen Antibodies  >164 publications with MIF and Spleen
 Liver Antibodies  >163 publications with MIF and Liver
 Heart Antibodies  >149 publications with MIF and Heart
 Lymph Antibodies  >129 publications with MIF and Lymph
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