Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit standard curve

Fractalkine/CX3CL1 (CX3CL1) ELISA Kit for Mouse

Name: Mouse CX3CL1 ELISA Kit; Fractalkine/CX3CL1; MyBioSource
Brand: MyBioSource
SKU: MBS175845
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS175845

Principle of the assay: mouse Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Fractalkine has been precoated onto 96-well plates. Standards(sf21, Q25-R337) and… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Fractalkine/CX3CL1, ELISA Kit

Full Product Name

Mouse Fractalkine/CX3CL1 PicoKine ELISA Kit

Product Gene Name

CX3CL1 elisa kit

Product Synonym Names

Fractalkine; chemokine (C-X3-C motif) ligand 1; A 152E5.2; AB030188; ABCD 3; ABCD3; C X3 C motif chemokine 1; C3Xkine; Chemokine (C X3 C motif) ligand 1; Chemokine C X3 C motif ligand 1; Chemokine CX3C Motif Ligand 1; CX3C membrane anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; NTN; NTT; SCYD 1; SCYD1; CX3XL1; Small inducible cytokine D1; Small inducible cytokine subfamily D (Cys X3 Cys), member 1; Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); Small inducible cytokine subfamily D member 1

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates

Detection Range

156pg/ml-10,000pg/ml

Sensitivity

< 10pg/ml

Specificity

Natural and recombinant mouse Fractalkine

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Intra-assay Precision

Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid inhumans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesiveand migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on humanchromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator ofsmooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5

Molecular Weight

42,203 Da

3D Structure

ModBase 3D Structure for A0N0N7

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CX3CL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CX3CL1 purchase

MBS175845 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fractalkine/CX3CL1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CX3CL1. The ELISA analytical biochemical technique of the MBS175845 kit is based on CX3CL1 antibody-CX3CL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CX3CL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CX3CL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CX3CL1. It may not necessarily be applicable to this product.

NCBI GeneID

6376

UniProt Primary Accession #

A0N0N7

UniProt Related Accession #

P78423

NCBI Official Synonym Full Names

chemokine (C-X3-C motif) ligand 1

NCBI Official Symbol

CX3CL1

NCBI Official Synonym Symbols

NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin

UniProt Protein Name

Chemokine (C-X3-C motif) ligand 1

UniProt Synonym Protein Names

Chemokine (C-X3-C motif) ligand 1

Protein Family

Fractalkine

UniProt Gene Name

CX3CL1

UniProt Entry Name

A0N0N7_HUMAN

NCBI Protein Information

fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)

UniProt Comments for CX3CL1

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; angiogenesis involved in wound healing; chemotaxis; macrophage chemotaxis; leukocyte adhesive activation; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response

Research Articles on CX3CL1

1. Low expression of CX3CL1 is gender-specifically associated with soft tissue sarcoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CX3CL1 elisa kit

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with CX3CL1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>322 publications with CX3CL1 and Inflammation
Necrosis Antibodies	>174 publications with CX3CL1 and Necrosis
Brain Diseases Antibodies	>91 publications with CX3CL1 and Brain Diseases
Atherosclerosis Antibodies	>84 publications with CX3CL1 and Atherosclerosis
Pain Antibodies	>56 publications with CX3CL1 and Pain
Kidney Diseases Antibodies	>55 publications with CX3CL1 and Kidney Diseases
Liver Diseases Antibodies	>46 publications with CX3CL1 and Liver Diseases
Heart Diseases Antibodies	>45 publications with CX3CL1 and Heart Diseases
Myocardial Ischemia Antibodies	>37 publications with CX3CL1 and Myocardial Ischemia
Fibrosis Antibodies	>32 publications with CX3CL1 and Fibrosis

Organs/Tissues associated with CX3CL1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>176 publications with CX3CL1 and Brain
Muscle Antibodies	>88 publications with CX3CL1 and Muscle
Kidney Antibodies	>83 publications with CX3CL1 and Kidney
Lung Antibodies	>80 publications with CX3CL1 and Lung
Heart Antibodies	>58 publications with CX3CL1 and Heart
Nerve Antibodies	>47 publications with CX3CL1 and Nerve
Intestine Antibodies	>32 publications with CX3CL1 and Intestine
Eye Antibodies	>26 publications with CX3CL1 and Eye
Embryonic Tissue Antibodies	>25 publications with CX3CL1 and Embryonic Tissue
Pancreas Antibodies	>18 publications with CX3CL1 and Pancreas