IGF-2 (IGF2) ELISA Kit for Rat

Principle of the Assay: The rat IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-2 has been precoated onto 90-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A25-E91) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biolin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGF-2 amount of sample captured in plate.

Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. 1 It is paternally expressed in the fetus and placenta. 2 IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele irherited from the father is active and the allele inhedted from the mother is inactive in most normal tissues.3 IGF-II appears to be induced by placental lactogen during prenatal development.4 It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.5

Small volumes of IGF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS175899 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IGF-2 (IGF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IGF2. The ELISA analytical biochemical technique of the MBS175899 kit is based on IGF2 antibody-IGF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IGF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IGF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).