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Hamartin (TSC1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176028

Background: TSC1(Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones… More details

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Product Overview

Online Datasheet in PDF
Product Name
Hamartin (TSC1), Polyclonal Antibody
Full Product Name
Anti-Hamartin antibody
Product Gene Name
anti-TSC1 antibody
Product Synonym Names
Hamartin; tuberous sclerosis 1; Hamartin antibody; kiaa0243 antibody; LAM antibody; TSC antibody; TSC1 antibody; Tsc1 gene antibody; TSC1_HUMAN antibody; Tuberous sclerosis 1 antibody; Tuberous sclerosis 1 protein antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with : Human, Rat
Predicted to work with : Mouse
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
124,015 Da
OMIM
gene 607341
3D Structure
ModBase 3D Structure for Q92574
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
MBS recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (please inquire) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (please inquire) for IHC(P).
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-TSC1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-TSC1 antibody
Western Blot (WB):
Concentration: 0.1-0.5ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse

Immunohistrochemistry (IHC) - Paraffin-embedded Section:
Concentration: 0.5-1ug/ml
Tested Species: Human
Predicted Species: Mouse,Rat
Antigen Retrieval: By Heat

WB: the detection limit for TSC1 is approximately 5ng/lane under reducing conditions.
Tested Species: in-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of fomalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-TSC1 antibody

Anti-Hamartin antibody, MBS176028, Western blotting
All lanes: Anti Hamartin (MBS176028) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 160KD
Rabbit Anti-TSC1 Polyclonal Antibody – Cat# MBS176028

Immunohistochemistry (IHC) of anti-TSC1 antibody

Anti-Hamartin antibody, MBS176028, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Rabbit Anti-TSC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176028

Immunohistochemistry (IHC) of anti-TSC1 antibody

Anti-Hamartin antibody, MBS176028, IHC(P)
IHC(P): Rat Brain Tissue
Rabbit Anti-TSC1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176028

Other Notes

Small volumes of anti-TSC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TSC1 purchase

MBS176028 is an antibody targeting TSC1. Also known as Anti-Hamartin antibody. Reactive in Reacts with : Human, Rat
Predicted to work with : Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TSC1. It may not necessarily be applicable to this product.

NCBI GI #
9297077
NCBI GeneID
7248
NCBI Accession #
Q92574.2
UniProt Primary Accession #
Q92574
UniProt Secondary Accession #
Q5VVN5; B7Z897
UniProt Related Accession #
Q92574
NCBI Official Full Name
Hamartin
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
TSC1
NCBI Official Synonym Symbols
LAM; TSC
UniProt Protein Name
Hamartin
UniProt Synonym Protein Names
Tuberous sclerosis 1 protein
Protein Family
Hamartin
UniProt Gene Name
TSC1
UniProt Synonym Gene Names
KIAA0243; TSC
UniProt Entry Name
TSC1_HUMAN
NCBI Protein Information
hamartin; tumor suppressor; tuberous sclerosis 1 protein

NCBI Summary for TSC1

This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]

UniProt Comments for TSC1

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: TSC1-TSC2 complex; protein complex; growth cone; intracellular membrane-bound organelle; membrane; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: protein binding; chaperone binding; protein N-terminus binding; GTPase activating protein binding; GTPase regulator activity

Biological Process: myelination; protein heterooligomerization; regulation of cell-matrix adhesion; regulation of cell cycle; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; cell projection organization and biogenesis; positive regulation of focal adhesion formation; synapse organization and biogenesis; kidney development; cell cycle arrest; potassium ion transport; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; negative regulation of translation; regulation of stress fiber formation; glucose import; neural tube closure; insulin receptor signaling pathway; cerebral cortex development; regulation of protein kinase activity

Disease: Lymphangioleiomyomatosis; Focal Cortical Dysplasia Of Taylor; Tuberous Sclerosis 1
Show more

Research Articles on TSC1

1. In a large family with the two disorders with two novel frameshift TSC1 and LMX1B mutations, we describe the phenotypes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TSC1 antibody

 Reference Product  PubMed Publications
 TSC2 antibody  >869 publications with TSC1 and TSC2
 MTOR antibody  >470 publications with TSC1 and MTOR
 RHEB antibody  >150 publications with TSC1 and RHEB
 AKT1 antibody  >29 publications with TSC1 and AKT1
 IKBKB antibody  >9 publications with TSC1 and IKBKB
 RPS6KA1 antibody  >5 publications with TSC1 and RPS6KA1
 YWHAB antibody  >1 publications with TSC1 and YWHAB

Pathways associated with anti-TSC1 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Pathway antibodies  IRS-related Events Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Inhibition Of TSC Complex Formation By PKB Pathway antibodies  Inhibition Of TSC Complex Formation By PKB Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with anti-TSC1 antibody

 Disease Name  Pubmed Publications
 Tuberous Sclerosis Antibodies  >975 publications with TSC1 and Tuberous Sclerosis
 Epilepsy Antibodies  >160 publications with TSC1 and Epilepsy
 Brain Diseases Antibodies  >125 publications with TSC1 and Brain Diseases
 Lymphangioleiomyomatosis Antibodies  >94 publications with TSC1 and Lymphangioleiomyomatosis
 Kidney Diseases Antibodies  >92 publications with TSC1 and Kidney Diseases
 Adenocarcinoma Antibodies  >48 publications with TSC1 and Adenocarcinoma
 Carcinoma, Renal Cell Antibodies  >38 publications with TSC1 and Carcinoma, Renal Cell
 Cardiovascular Diseases Antibodies  >36 publications with TSC1 and Cardiovascular Diseases
 Tuberous Sclerosis 1 Antibodies  >35 publications with TSC1 and Tuberous Sclerosis 1
 Neoplasms, Experimental Antibodies  >30 publications with TSC1 and Neoplasms, Experimental

Organs/Tissues associated with anti-TSC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >306 publications with TSC1 and Brain
 Kidney Antibodies  >156 publications with TSC1 and Kidney
 Lung Antibodies  >109 publications with TSC1 and Lung
 Blood Antibodies  >98 publications with TSC1 and Blood
 Skin Antibodies  >98 publications with TSC1 and Skin
 Heart Antibodies  >90 publications with TSC1 and Heart
 Muscle Antibodies  >85 publications with TSC1 and Muscle
 Liver Antibodies  >54 publications with TSC1 and Liver
 Vascular Antibodies  >45 publications with TSC1 and Vascular
 Bladder Antibodies  >28 publications with TSC1 and Bladder
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