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SLC12A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176766

Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is… More details

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Product Overview

Online Datasheet in PDF
Product Name
NKCC1 (SLC12A2), Polyclonal Antibody
Full Product Name
Anti-NKCC1 antibody
Product Gene Name
anti-SLC12A2 antibody
Product Synonym Names
Solute carrier family 12 member 2; solute carrier family 12(sodium/potassium/chloride transporters), member 2; Basolateral Na-K-Cl symporter antibody; BSC antibody; BSC2 antibody; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 antibody; mBSC2 antibody; MGC104233 antibody; NKCC1 antibody; S12A2_HUMAN antibody; SLC12A2 antibody; sodium-potassium-chloride cotransporter 1 antibody; solute carrier family 12(sodium/potassium/chloride transporters) member 2 antibody; Solute carrier family 12 member 2 antibody; sy-ns antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Form/Format
Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
600840
3D Structure
ModBase 3D Structure for P55011
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A2 (223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-SLC12A2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen Section.
Application Notes for anti-SLC12A2 antibody
Western Blot:
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Immunohistochemistry (Frozen Section):
Concentration: 0.5-1 ug/ml
Tested Species: Rat
Predicted Species: Hu, Ms

Tested Species: In-House tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins, is required for the staining of formalin/paraffin sections.

Other applications have not been tested. Optimal dilutions should be determined by end user.

Other Notes

Small volumes of anti-SLC12A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC12A2 purchase

MBS176766 is an antibody targeting SLC12A2. Also known as Anti-NKCC1 antibody. Reactive in Reacts with: Human, Rat
Predicted to work with: Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC12A2. It may not necessarily be applicable to this product.

NCBI GI #
1709292
NCBI GeneID
6558
NCBI Accession #
P55011.1
UniProt Primary Accession #
P55011
UniProt Secondary Accession #
Q8N713; Q8WWH7
UniProt Related Accession #
P55011
NCBI Official Full Name
Solute carrier family 12 member 2
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/potassium/chloride transporter), member 2
NCBI Official Symbol
SLC12A2
NCBI Official Synonym Symbols
BSC; BSC2; NKCC1; PPP1R141
UniProt Protein Name
Solute carrier family 12 member 2
UniProt Synonym Protein Names
Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
Protein Family
Solute carrier family
UniProt Gene Name
SLC12A2
UniProt Synonym Gene Names
NKCC1
UniProt Entry Name
S12A2_HUMAN
NCBI Protein Information
solute carrier family 12 member 2; basolateral Na-K-Cl symporter; protein phosphatase 1, regulatory subunit 141; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; solute carrier family 12 (sodium/potassium/chloride transporters), member 2

NCBI Summary for SLC12A2

The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for SLC12A2

NKCC1: a multi-pass membrane cation transporter protein. Aids transcellular movement of chloride across both secretory and absorptive epithelia. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Expressed in many tissues. Bumetanide is a potent NKCC1 antagonist. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 5q23.3

Cellular Component: membrane; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; vesicle

Molecular Function: protein binding; ammonium transmembrane transporter activity; sodium:potassium:chloride symporter activity

Biological Process: hyperosmotic response; transport; transepithelial chloride transport; multicellular organism growth; sodium ion transport; ion transport; detection of mechanical stimulus involved in sensory perception of sound; transmembrane transport; positive regulation of cell volume; ammonium transport; gamma-aminobutyric acid signaling pathway; potassium ion transport
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Research Articles on SLC12A2

1. Functional expression of human NKCC1 from a synthetic cassette-based cDNA: introduction of extracellular epitope tags and removal of cysteines.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC12A2 antibody

 Reference Product  PubMed Publications
 KCNQ1 antibody  >11 publications with SLC12A2 and KCNQ1
 WNK3 antibody  >7 publications with SLC12A2 and WNK3
 OXSR1 antibody  >5 publications with SLC12A2 and OXSR1
 EGF antibody  >3 publications with SLC12A2 and EGF
 JAK2 antibody  >2 publications with SLC12A2 and JAK2

Pathways associated with anti-SLC12A2 antibody

 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 Pancreatic Secretion Pathway antibodies  Pancreatic Secretion Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Salivary Secretion Pathway antibodies  Salivary Secretion Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
 Vibrio Cholerae Infection Pathway antibodies  Vibrio Cholerae Infection Pathway Diagram
 Vibrio Cholerae Infection Pathway antibodies  Vibrio Cholerae Infection Pathway Diagram

Diseases associated with anti-SLC12A2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >90 publications with SLC12A2 and Nervous System Diseases
 Brain Diseases Antibodies  >61 publications with SLC12A2 and Brain Diseases
 Epilepsy Antibodies  >37 publications with SLC12A2 and Epilepsy
 Cardiovascular Diseases Antibodies  >35 publications with SLC12A2 and Cardiovascular Diseases
 Hypertension Antibodies  >25 publications with SLC12A2 and Hypertension
 Disease Models, Animal Antibodies  >23 publications with SLC12A2 and Disease Models, Animal
 Kidney Diseases Antibodies  >19 publications with SLC12A2 and Kidney Diseases
 Edema Antibodies  >17 publications with SLC12A2 and Edema
 Brain Edema Antibodies  >14 publications with SLC12A2 and Brain Edema
 Inflammation Antibodies  >13 publications with SLC12A2 and Inflammation

Organs/Tissues associated with anti-SLC12A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >159 publications with SLC12A2 and Brain
 Kidney Antibodies  >61 publications with SLC12A2 and Kidney
 Blood Antibodies  >52 publications with SLC12A2 and Blood
 Intestine Antibodies  >34 publications with SLC12A2 and Intestine
 Muscle Antibodies  >32 publications with SLC12A2 and Muscle
 Vascular Antibodies  >31 publications with SLC12A2 and Vascular
 Ear Antibodies  >28 publications with SLC12A2 and Ear
 Spinal Cord Antibodies  >20 publications with SLC12A2 and Spinal Cord
 Lung Antibodies  >19 publications with SLC12A2 and Lung
 Liver Antibodies  >19 publications with SLC12A2 and Liver
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