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CMA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176889

Description: Rabbit IgG polyclonal antibody for Chymase(CMA1) detection. Tested with WB, IHC-P in Human.Background: Chymase is a major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and… More details

$210 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
CMA1, Polyclonal Antibody
Full Product Name
Anti-CMA1 Antibody
Product Gene Name
anti-CMA1 antibody
Product Synonym Names
Chymase; chymase 1, mast cell; Alpha-chymase antibody; Chymase 1 antibody; Chymase 1 mast cell antibody; Chymase antibody; Chymase, heart antibody; Chymase, mast cell antibody; CMA1 antibody; CMA1_HUMAN antibody; CYH antibody; CYM antibody; EC 3.4.21.39 antibody; Mast cell chymase 1 antibody; Mast cell protease 3 antibody; Mast cell protease 5 antibody; Mast cell protease I antibody; Mast cell protease III antibody; Mcp-5 antibody; MCP3P antibody; Mcpt5 antibody; MGC119890 antibody; MGC119891 antibody; MMCP-5 antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Reacts with Human. No cross reactivity with other proteins
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
118938
3D Structure
ModBase 3D Structure for P23946
Immunogen
E Coli-derived human CMA1 recombinant protein (Position: I22-N247). Human CMA1 shares 75% and 74% amino acid (aa) sequence identity with mouse and rat CMA1, respectively.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CMA1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CMA1 antibody
Western Blot:

Concentration: 0.1-0.5 ug/ml
Tested Species: Human


IHC (Paraffin-embedded Section):

Concentration: 0.5-1 ug/ml
Tested Species: Human
Antigen Retrieval: By Heat



WB: The detection limit for CMA1 is approximately 0.25ng/lane under reducing conditions.

Tested Species: In-house tested species with positive results.

By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Other applications not tested.

Optimal dilutions should be determined by the end users.

Immunohistochemistry (IHC) of anti-CMA1 antibody

Anti-CMA1 Picoband antibody, MBS176889
IHC(P): Human Placenta Tissue
Rabbit Anti-CMA1 Polyclonal Antibody – Cat# MBS176889

Western Blot (WB) of anti-CMA1 antibody

Western blot analysis of CMA1 using anti- CMA1 antibody (MBS176889).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CMA1 antigen affinity purified polyclonal antibody (Catalog # MBS176889) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD.
Rabbit Anti-CMA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS176889

Other Notes

Small volumes of anti-CMA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CMA1 purchase

MBS176889 is an antibody targeting CMA1. Reactive in Reacts with Human. No cross reactivity with other proteins. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CMA1. It may not necessarily be applicable to this product.

NCBI GI #
126825
NCBI GeneID
1215
NCBI Accession #
P23946.1
UniProt Primary Accession #
P23946
UniProt Secondary Accession #
Q16018; Q3SY36; Q3SY37; Q9UDH5; B5BUM8
UniProt Related Accession #
P23946
NCBI Official Full Name
Chymase
NCBI Official Synonym Full Names
chymase 1, mast cell
NCBI Official Symbol
CMA1
NCBI Official Synonym Symbols
CYH; MCT1; chymase
UniProt Protein Name
Chymase
UniProt Synonym Protein Names
Alpha-chymase; Mast cell protease I
Protein Family
Chymase
UniProt Gene Name
CMA1
UniProt Synonym Gene Names
CYH; CYM
UniProt Entry Name
CMA1_HUMAN
NCBI Protein Information
chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I

NCBI Summary for CMA1

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]
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UniProt Comments for CMA1

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.39; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding

Biological Process: interleukin-1 beta biosynthetic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of angiogenesis; cellular protein metabolic process; regulation of inflammatory response; midbrain development; protein processing; proteolysis; angiotensin maturation
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Research Articles on CMA1

1. CMA1 gene single nucleotide polymorphisms is associated with essential hypertension.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CMA1 antibody

 Reference Product  PubMed Publications
 AGT antibody  >6 publications with CMA1 and AGT
 REN antibody  >2 publications with CMA1 and REN
 ACE2 antibody  >1 publications with CMA1 and ACE2
 KITLG antibody  >1 publications with CMA1 and KITLG

Pathways associated with anti-CMA1 antibody

 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with anti-CMA1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >11 publications with CMA1 and Inflammation
 Lung Diseases Antibodies  >10 publications with CMA1 and Lung Diseases
 Fibrosis Antibodies  >9 publications with CMA1 and Fibrosis
 Heart Diseases Antibodies  >8 publications with CMA1 and Heart Diseases
 Hypertension Antibodies  >8 publications with CMA1 and Hypertension
 Hypertrophy Antibodies  >5 publications with CMA1 and Hypertrophy
 Necrosis Antibodies  >4 publications with CMA1 and Necrosis
 Pulmonary Fibrosis Antibodies  >2 publications with CMA1 and Pulmonary Fibrosis
 Hypertrophy, Left Ventricular Antibodies  >2 publications with CMA1 and Hypertrophy, Left Ventricular
 Uterine Cervical Neoplasms Antibodies  >1 publications with CMA1 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-CMA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >1 publications with CMA1 and Mixed
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