Ve-Cadherin/CD144 (CDH5) ELISA Kit for Mouse

Principle of the assay: mouse VE-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VE-Cadherin has been precoated onto 96-well plates. Standards(NSO, D46-Q599) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VE-Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VE-Cadherin amount of sample captured in plate.
Background: Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.

Small volumes of CDH5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177141 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the VE-Cadherin/CD144 (CDH5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CDH5. The ELISA analytical biochemical technique of the MBS177141 kit is based on CDH5 antibody-CDH5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CDH5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CDH5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).