ADAMTS1 ELISA Kit for Human

Principle of the Assay: Human ADAMTS1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS1 amount of sample captured in plate.

Background/Introduction: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What's more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

Small volumes of ADAMTS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177144 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAMTS1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAMTS1. The ELISA analytical biochemical technique of the MBS177144 kit is based on ADAMTS1 antibody-ADAMTS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAMTS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAMTS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).