CCL8/MCP2 (CCL8) ELISA Kit for Mouse

Background: Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Principle of the Assay: The Picokine™ Mouse Ccl8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ccl8 with a 96-well strip plate that is pre-coated with antibody specific for Ccl8. The detection antibody is a biotinylated antibody specific for Ccl8. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse Ccl8 protein as standards. To measure Mouse Ccl8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ccl8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ccl8 in the sample.

Small volumes of CCL8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177281 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CCL8/MCP2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCL8. The ELISA analytical biochemical technique of the MBS177281 kit is based on CCL8 antibody-CCL8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCL8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCL8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).