TIMP-4 (TIMP4) ELISA Kit for Human

Principle of the assay: human TIMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-4 has been precoated onto 96-well plates. Standards(sf21, C30-P224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TIMP-4 amount of sample captured in plate.
Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Small volumes of TIMP4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177395 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TIMP-4 (TIMP4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TIMP4. The ELISA analytical biochemical technique of the MBS177395 kit is based on TIMP4 antibody-TIMP4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TIMP4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TIMP4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).