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Flt-3ligand (FLT3LG) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177397

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Flt-3ligand (FLT3LG), ELISA Kit
Full Product Name
Mouse Flt-3ligand PicoKine ELISA Kit
Product Gene Name
Product Synonym Names
Fms-related tyrosine kinase 3 ligand; fms-related tyrosine kinase 3 ligand; Flt 3 ligand; Flt 3L; Flt3 L; FLT3 LG; Flt3 ligand; Flt3L; FLT3L_HUMAN; Flt3lg; Fms related tyrosine kinase 3 ligand; Fms-related tyrosine kinase 3 ligand; SL cytokine
Species Reactivity
Mouse
Assay Type
Competitive
Samples
Serum, plasma, tissue homogenates
Detection Range
0.1 ng/ml-20 ng/ml
Sensitivity
< 0.05 ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse testosterone. No significant cross-reactivity or interference between mouse testosterone and analogues was observed.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Molecular Weight
28,259 Da
3D Structure
ModBase 3D Structure for P49772
Production Time
Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of FLT3LG elisa kit

Mouse Flt-3ligand PicoKine ELISA Kit standard curve
Mouse FLT3LG ELISA Kit – Cat# MBS177397

Other Notes

Small volumes of FLT3LG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FLT3LG purchase

MBS177397 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Flt-3ligand (FLT3LG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLT3LG. The ELISA analytical biochemical technique of the MBS177397 kit is based on FLT3LG antibody-FLT3LG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLT3LG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLT3LG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FLT3LG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Fms-related tyrosine kinase 3 ligand
NCBI Official Synonym Full Names
FMS-like tyrosine kinase 3 ligand
NCBI Official Symbol
Flt3l
NCBI Official Synonym Symbols
Ly72L; Flt3lg
UniProt Protein Name
Fms-related tyrosine kinase 3 ligand
UniProt Synonym Protein Names
SL cytokine
UniProt Gene Name
Flt3lg
UniProt Synonym Gene Names
Flt3l; Flt3 ligand; Flt3L
UniProt Entry Name
FLT3L_MOUSE
NCBI Protein Information
fms-related tyrosine kinase 3 ligand; flt3 ligand

UniProt Comments for FLT3LG

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Cytokine; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding

Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of myoblast differentiation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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