Mouse TFPI PicoKine ELISA Kit standard curve

Tissue Factor Pathway Inhibitor (TFPI) ELISA Kit for Mouse

Name: Mouse TFPI ELISA Kit; Tissue Factor Pathway Inhibitor; MyBioSource
Brand: MyBioSource
SKU: MBS177538
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177538

Description: Sandwich ELISA kit of Quantitative Detection for Mouse TFPIBackground: TFPI(Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa).… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Tissue factor pathway inhibitor (TFPI), ELISA Kit

Full Product Name

Mouse TFPI PicoKine ELISA Kit

Product Gene Name

TFPI elisa kit

Product Synonym Names

Anti convertin; EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein associated coagulation inhibitor; Lipoprotein-associated coagulation inhibitor; TFI; TFPI 1; TFPI; TFPI1; TFPI1_HUMAN; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor); Tissue factor pathway inhibitor; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

Species Reactivity

Mouse. There is no detectable cross-reactivity with other relevant proteins.

Assay Type

Sandwich

Samples

Cell culture supernates, serum and plasma (heparin, EDTA)

Sensitivity

<10pg/ml

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Molecular Weight

28,949 Da

3D Structure

ModBase 3D Structure for O54819

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Immunogen

NSO, L29-N306

Intra-Assay Precision

Sample 1: n=16; Mean(ng/ml): 1.05; Standard deviation: 0.048; CV(%): 4.6 Sample 2: n=16; Mean(ng/ml): 2.91; Standard deviation: 0.122; CV(%): 4.2 Sample 3: n=16; Mean(ng/ml): 5.34; Standard deviation: 0.203; CV(%): 3.8

Inter-Assay Precision

Sample 1: n=24; Mean(ng/ml): 1.21; Standard deviation: 0.076; CV(%): 6.3 Sample 2: n=24; Mean(ng/ml): 3.45; Standard deviation: 0.193; CV(%): 5.6 Sample 3: n=24; Mean(ng/ml): 5.82; Standard deviation: 0.285; CV(%): 4.9

Assay Range

156pg/ml-10,000pg/ml

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of TFPI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI purchase

MBS177538 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tissue factor pathway inhibitor (TFPI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TFPI. The ELISA analytical biochemical technique of the MBS177538 kit is based on TFPI antibody-TFPI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TFPI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TFPI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI. It may not necessarily be applicable to this product.

NCBI GI #

33859644

NCBI GeneID

21788

NCBI Accession #

NP_035706.1

UniProt Primary Accession #

O54819

UniProt Secondary Accession #

Q9Z2U8

UniProt Related Accession #

O54819

GenBank Nucleotide #

NM_011576.1

NCBI Official Full Name

tissue factor pathway inhibitor isoform a

NCBI Official Synonym Full Names

tissue factor pathway inhibitor

NCBI Official Symbol

Tfpi

NCBI Official Synonym Symbols

EPI; LACI; AW552122; A630013F22Rik

UniProt Protein Name

Tissue factor pathway inhibitor

UniProt Synonym Protein Names

Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI

Protein Family

Tissue factor pathway inhibitor

UniProt Gene Name

Tfpi

UniProt Synonym Gene Names

TFPI; EPI; LACI

UniProt Entry Name

TFPI1_MOUSE

NCBI Protein Information

tissue factor pathway inhibitor; extrinsic pathway inhibitor; lipoprotein-associated coagulation inhibitor

UniProt Comments for TFPI

TFPI: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Secreted; Inhibitor; Secreted, signal peptide

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protease inhibitor activity

Biological Process: hemostasis; negative regulation of peptidase activity; blood coagulation

Research Articles on TFPI

1. The increased plasmin generation during the early stages of sepsis could cleave/inactivate TFPI and thus lead to thrombotic complications.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TFPI elisa kit

Products by Pathway	Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Extrinsic Pathway antibodies	Extrinsic Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with TFPI elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>530 publications with TFPI and Cardiovascular Diseases
Inflammation Antibodies	>196 publications with TFPI and Inflammation
Heart Diseases Antibodies	>133 publications with TFPI and Heart Diseases
Venous Thrombosis Antibodies	>129 publications with TFPI and Venous Thrombosis
Carcinoma Antibodies	>80 publications with TFPI and Carcinoma
Necrosis Antibodies	>78 publications with TFPI and Necrosis
Disease Models, Animal Antibodies	>77 publications with TFPI and Disease Models, Animal
Myocardial Infarction Antibodies	>66 publications with TFPI and Myocardial Infarction
Kidney Diseases Antibodies	>58 publications with TFPI and Kidney Diseases
Neoplasm Metastasis Antibodies	>39 publications with TFPI and Neoplasm Metastasis

Organs/Tissues associated with TFPI elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>102 publications with TFPI and Embryonic Tissue
Lung Antibodies	>89 publications with TFPI and Lung
Heart Antibodies	>85 publications with TFPI and Heart
Kidney Antibodies	>80 publications with TFPI and Kidney
Liver Antibodies	>79 publications with TFPI and Liver
Brain Antibodies	>41 publications with TFPI and Brain
Bone Antibodies	>18 publications with TFPI and Bone
Skin Antibodies	>17 publications with TFPI and Skin
Ovary Antibodies	>17 publications with TFPI and Ovary
Bone Marrow Antibodies	>16 publications with TFPI and Bone Marrow