Human Biglycan PicoKine ELISA Kit standard curve

Biglycan (BGN) ELISA Kit for Human

Name: Human BGN ELISA Kit; Biglycan; MyBioSource
Brand: MyBioSource
SKU: MBS177549
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177549

Description: Sandwich ELISA kit of Quantitative Detection for Human BiglycanBackground: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Biglycan (BGN), ELISA Kit

Full Product Name

Human Biglycan PicoKine ELISA Kit

Product Gene Name

BGN elisa kit

Product Synonym Names

BGN; Biglycan; Biglycan proteoglycan; Bone/cartilage proteoglycan I; Dermatan sulphate proteoglycan I; DSPG1; PG S1; PG-S1; PGI; PGS1_HUMAN; SLRR1A; Small leucine rich protein 1A; biglycan

Species Reactivity

Human. There is no detectable cross-reactivity with other relevant proteins.

Assay Type

Sandwich

Samples

Cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates

Sensitivity

<10pg/ml

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Molecular Weight

41,654 Da

OMIM

301870

3D Structure

ModBase 3D Structure for P21810

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Immunogen

NSO, D38-K368

Intra-Assay Precision

Sample 1: n=16; Mean(ng/ml): 1.62; Standard deviation: 0.06; CV(%): 3.7 Sample 2: n=16; Mean(ng/ml): 2.91; Standard deviation: 0.157; CV(%): 5.4 Sample 3: n=16; Mean(ng/ml): 5.38; Standard deviation: 0.264; CV(%): 4.9

Inter-Assay Precision

Sample 1: n=24; Mean(ng/ml): 1.52; Standard deviation: 0.062; CV(%): 4.1 Sample 2: n=24; Mean(ng/ml): 3.07; Standard deviation: 0.209; CV(%): 6.8 Sample 3: n=24; Mean(ng/ml): 6.34; Standard deviation: 0.33; CV(%): 5.2

Assay Range

156pg/ml-10,000pg/ml

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of BGN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BGN purchase

MBS177549 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Biglycan (BGN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BGN. The ELISA analytical biochemical technique of the MBS177549 kit is based on BGN antibody-BGN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BGN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BGN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BGN. It may not necessarily be applicable to this product.

NCBI GI #

4502403

NCBI GeneID

633

NCBI Accession #

NP_001702.1

UniProt Primary Accession #

P21810

UniProt Secondary Accession #

P13247; D3DWU3

UniProt Related Accession #

P21810

GenBank Nucleotide #

NM_001711.4

NCBI Official Full Name

biglycan preproprotein

NCBI Official Synonym Full Names

biglycan

NCBI Official Symbol

BGN

NCBI Official Synonym Symbols

PGI; DSPG1; PG-S1; SLRR1A

UniProt Protein Name

Biglycan

UniProt Synonym Protein Names

Bone/cartilage proteoglycan I; PG-S1

Protein Family

Biglycan

UniProt Gene Name

BGN

UniProt Synonym Gene Names

SLRR1A

UniProt Entry Name

PGS1_HUMAN

NCBI Protein Information

biglycan; biglycan proteoglycan; bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; small leucine-rich protein 1A; dermatan sulphate proteoglycan I

NCBI Summary for BGN

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The encoded protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein plays a role in assembly of collagen fibrils and muscle regeneration. It interacts with several proteins involved in muscular dystrophy, including alpha-dystroglycan, alpha- and gamma-sarcoglycan and collagen VI, and it is critical for the assembly of the dystrophin-associated protein complex. [provided by RefSeq, Nov 2009]

UniProt Comments for BGN

BGN: May be involved in collagen fiber assembly. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; lysosomal lumen; transport vesicle; cell surface; Golgi lumen; extracellular region; sarcolemma

Molecular Function: glycosaminoglycan binding; extracellular matrix binding; extracellular matrix structural constituent

Biological Process: chondroitin sulfate metabolic process; extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; chondroitin sulfate catabolic process; carbohydrate metabolic process; blood vessel remodeling; pathogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; dermatan sulfate biosynthetic process

Research Articles on BGN

1. Data indicate that the biglycan (BGN) CC genotype was significantly over-represented in the control group versus the carpal tunnel syndrome (CTS) group.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BGN elisa kit

Reference Product	PubMed Publications
VCAN elisa kit	>45 publications with BGN and VCAN
TNF elisa kit	>7 publications with BGN and TNF
SDC1 elisa kit	>6 publications with BGN and SDC1
BMP4 elisa kit	>5 publications with BGN and BMP4
SDC2 elisa kit	>3 publications with BGN and SDC2
GPC1 elisa kit	>1 publications with BGN and GPC1
BCAN elisa kit	>1 publications with BGN and BCAN

Pathways associated with BGN elisa kit

Products by Pathway	Pathway Diagram
A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies	A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
CS/DS Degradation Pathway antibodies	CS/DS Degradation Pathway Diagram
Chondroitin Sulfate Biosynthesis Pathway antibodies	Chondroitin Sulfate Biosynthesis Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Defective B3GAT3 Causes JDSSDHD Pathway antibodies	Defective B3GAT3 Causes JDSSDHD Pathway Diagram
Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway antibodies	Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway Diagram
Defective B4GALT7 Causes EDS, Progeroid Type Pathway antibodies	Defective B4GALT7 Causes EDS, Progeroid Type Pathway Diagram
Defective CHST14 Causes EDS, Musculocontractural Type Pathway antibodies	Defective CHST14 Causes EDS, Musculocontractural Type Pathway Diagram
Defective CHST3 Causes SEDCJD Pathway antibodies	Defective CHST3 Causes SEDCJD Pathway Diagram
Defective CHST6 Causes MCDC1 Pathway antibodies	Defective CHST6 Causes MCDC1 Pathway Diagram

Diseases associated with BGN elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>33 publications with BGN and Fibrosis
Disease Models, Animal Antibodies	>30 publications with BGN and Disease Models, Animal
Inflammation Antibodies	>29 publications with BGN and Inflammation
Necrosis Antibodies	>22 publications with BGN and Necrosis
Kidney Diseases Antibodies	>21 publications with BGN and Kidney Diseases
Carcinoma Antibodies	>11 publications with BGN and Carcinoma
Arthritis, Rheumatoid Antibodies	>10 publications with BGN and Arthritis, Rheumatoid
Hypertension Antibodies	>6 publications with BGN and Hypertension
Hyperplasia Antibodies	>5 publications with BGN and Hyperplasia
Liver Diseases Antibodies	>5 publications with BGN and Liver Diseases

Organs/Tissues associated with BGN elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>133 publications with BGN and Bone
Connective Tissue Antibodies	>90 publications with BGN and Connective Tissue
Vascular Antibodies	>76 publications with BGN and Vascular
Muscle Antibodies	>76 publications with BGN and Muscle
Blood Antibodies	>55 publications with BGN and Blood
Mouth Antibodies	>41 publications with BGN and Mouth
Kidney Antibodies	>38 publications with BGN and Kidney
Skin Antibodies	>38 publications with BGN and Skin
Lung Antibodies	>28 publications with BGN and Lung
Heart Antibodies	>28 publications with BGN and Heart