Mouse COL18A1 ELISA Kit – Cat# MBS177551

Mouse Endostatin PicoKine ELISA Kit standard curve

Collagen Alpha-1(xviii) Chain (COL18A1) ELISA Kit for Mouse

Name: Mouse COL18A1 ELISA Kit; Collagen Alpha-1(xviii) Chain; MyBioSource
Brand: MyBioSource
SKU: MBS177551
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177551

Description: Sandwich ELISA kit of Quantitative Detection for Mouse EndostatinBackground: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Collagen alpha-1(XVIII) chain (COL18A1), ELISA Kit

Full Product Name

Mouse Endostatin PicoKine ELISA Kit

Product Gene Name

COL18A1 elisa kit

Product Synonym Names

Alpha 1 collagen type 18 (XVIII)(COL18A1); Alpha 1 type XVIII collagen; Antiangiogenic agent; COIA1_HUMAN; COL15A1; COL18A1; Collagen alpha 1(XV) chain; Collagen alpha 1(XVIII) chain; Collagen alpha-1(XV) chain; Collagen type XV proteoglycan; Collagen type XVIII alpha 1; Collagen XV, alpha 1 polypeptide; Collagen, type XV, alpha 1; Endostatin; Endostatin XV; FLJ27325; FLJ34914; FLJ38566; KNO; KNO1; KS; MGC74745; Multi functional protein MFP; OTTHUMP00000021782; OTTHUMP00000115472; OTTHUMP00000115473; collagen, type XVIII, alpha 1

Species Reactivity

Mouse. There is no detectable cross-reactivity with other relevant proteins.

Assay Type

Sandwich

Samples

Cell culture supernates, serum and plasma (heparin, EDTA)

Sensitivity

<10pg/ml

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Molecular Weight

134,204 Da

3D Structure

ModBase 3D Structure for P39061

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Immunogen

NSO, H1591-K1774

Intra-Assay Precision

Sample 1: n=16; Mean(ng/ml): 0.8; Standard deviation: 0.052; CV(%): 6.5 Sample 2: n=16; Mean(ng/ml): 2.14; Standard deviation: 0.135; CV(%): 6.3 Sample 3: n=16; Mean(ng/ml): 4.25; Standard deviation: 0.179; CV(%): 4.2

Inter-Assay Precision

Sample 1: n=24; Mean(ng/ml): 0.84; Standard deviation: 0.06; CV(%): 7.1 Sample 2: n=24; Mean(ng/ml): 2.23; Standard deviation: 0.147; CV(%): 6.6 Sample 3: n=24; Mean(ng/ml): 4.37; Standard deviation: 0.253; CV(%): 5.8

Assay Range

156pg/ml-10,000pg/ml

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of COL18A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COL18A1 purchase

MBS177551 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Collagen alpha-1(XVIII) chain (COL18A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COL18A1. The ELISA analytical biochemical technique of the MBS177551 kit is based on COL18A1 antibody-COL18A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COL18A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COL18A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for COL18A1. It may not necessarily be applicable to this product.

NCBI GI #

158508560

NCBI GeneID

12822

NCBI Accession #

NP_001103461.1

UniProt Primary Accession #

P39061

UniProt Secondary Accession #

Q60672; Q61434; Q61437; Q62001; Q62002; Q6NZK9; Q6P1Y4; Q8CCZ8; Q9CRT2; Q9JK63

UniProt Related Accession #

P39061

GenBank Nucleotide #

NM_001109991.1

NCBI Official Full Name

collagen alpha-1(XVIII) chain isoform 1

NCBI Official Synonym Full Names

collagen, type XVIII, alpha 1

NCBI Official Symbol

Col18a1

UniProt Protein Name

Collagen alpha-1(XVIII) chain

Protein Family

Collagen

UniProt Gene Name

Col18a1

UniProt Entry Name

COIA1_MOUSE

NCBI Protein Information

collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1

UniProt Comments for COL18A1

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane

Molecular Function: identical protein binding; metal ion binding; structural molecule activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; positive regulation of cell migration

Research Articles on COL18A1

1. Specific collagen XVIII isoforms promote adipose tissue accrual via mechanisms determining

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with COL18A1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram
Laminin Interactions Pathway antibodies	Laminin Interactions Pathway Diagram
PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies	PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram

Diseases associated with COL18A1 elisa kit

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>150 publications with COL18A1 and Lung Neoplasms
Liver Neoplasms Antibodies	>119 publications with COL18A1 and Liver Neoplasms
Liver Diseases Antibodies	>116 publications with COL18A1 and Liver Diseases
Neoplasm Metastasis Antibodies	>103 publications with COL18A1 and Neoplasm Metastasis
Brain Diseases Antibodies	>71 publications with COL18A1 and Brain Diseases
Breast Neoplasms Antibodies	>54 publications with COL18A1 and Breast Neoplasms
Necrosis Antibodies	>52 publications with COL18A1 and Necrosis
Carcinoma, Hepatocellular Antibodies	>51 publications with COL18A1 and Carcinoma, Hepatocellular
Liver Neoplasms, Experimental Antibodies	>48 publications with COL18A1 and Liver Neoplasms, Experimental
Kidney Diseases Antibodies	>47 publications with COL18A1 and Kidney Diseases

Organs/Tissues associated with COL18A1 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>212 publications with COL18A1 and Lung
Liver Antibodies	>164 publications with COL18A1 and Liver
Eye Antibodies	>130 publications with COL18A1 and Eye
Embryonic Tissue Antibodies	>98 publications with COL18A1 and Embryonic Tissue
Brain Antibodies	>83 publications with COL18A1 and Brain
Kidney Antibodies	>81 publications with COL18A1 and Kidney
Muscle Antibodies	>69 publications with COL18A1 and Muscle
Bone Antibodies	>62 publications with COL18A1 and Bone
Skin Antibodies	>53 publications with COL18A1 and Skin
Heart Antibodies	>41 publications with COL18A1 and Heart