Protein S100-A9 (S100A9) ELISA Kit for Mouse

Principle of the Assay: Mouse S100A9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for S100A9 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A2-K113) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for S100A9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse S100A9 amount of sample captured in plate.

Background/Introduction: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin- B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.

Small volumes of S100A9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177553 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein S100-A9 (S100A9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S100A9. The ELISA analytical biochemical technique of the MBS177553 kit is based on S100A9 antibody-S100A9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S100A9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S100A9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).