Mouse XCL1/Lymphotactin PicoKine ELISA Kit standard curve

XCL1/Lymphotactin Picokine (XCL1) ELISA Kit for Mouse

Name: Mouse XCL1 ELISA Kit; XCL1/Lymphotactin Picokine; MyBioSource
Brand: MyBioSource
SKU: MBS177584
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177584

Principle of the assay: Mouse XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for XCL1 has been precoated onto 96-well plates. Standards (E Coli, V22-G114) and test samples… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

XCL1/Lymphotactin PicoKine, ELISA Kit

Full Product Name

Mouse XCL1/Lymphotactin PicoKine ELISA Kit

Product Gene Name

Xcl1 elisa kit

Product Synonym Names

Lymphotactin; ATAC; C motif chemokine 1; Chemokine (C motif) ligand 1; Cytokine SCM-1; Cytokine SCM1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM 1; SCM 1 alpha; SCM-1-alpha; SCM1; SCM1 alpha; SCYC1; Small inducible cytokine C1; Small inducible cytokine subfamily C member 1; Small-inducible cytokine C1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN; chemokine (C motif) ligand 1

Species Reactivity

Mouse

Sensitivity

<5pg/ml

Specificity

Natural and recombinant mouse XCL1

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Molecular Weight

12,467 Da

3D Structure

ModBase 3D Structure for P47993

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Assay Range

62.5pg/ml-4000pg/ml

Sample

For quantitative detection of mouse XCL1 in cell culture supernates, serum and plasma(heparin, EDTA).

Immunogen

E Coli, V22-G114

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse XCL1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse XCL1 standard solution and each sample be measured in duplicate.

Intra-Assay Precision

Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|447|1253|2346|450|1238|2625
Standard deviation|28.61|53.88|115|39.15|64.38|144.4
CV(%)|6.4|4.3|4.9|8.7|5.2|5.5

Sample Data

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.004|0.036|0.071|0.147|0.321|0.733|1.673|2.409

Anticoagulant

Heparin or EDTA

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of Xcl1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Xcl1 purchase

MBS177584 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the XCL1/Lymphotactin PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Xcl1. The ELISA analytical biochemical technique of the MBS177584 kit is based on Xcl1 antibody-Xcl1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Xcl1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Xcl1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Xcl1. It may not necessarily be applicable to this product.

NCBI GI #

6678712

NCBI GeneID

16963

NCBI Accession #

NP_032536.1

UniProt Primary Accession #

P47993

UniProt Related Accession #

P47993

GenBank Nucleotide #

NM_008510.1

NCBI Official Full Name

lymphotactin

NCBI Official Synonym Full Names

chemokine (C motif) ligand 1

NCBI Official Symbol

Xcl1

NCBI Official Synonym Symbols

LTN; ATAC; Lptn; SCM-1; Scyc1; SCM-1a; AI661682

UniProt Protein Name

Lymphotactin

UniProt Synonym Protein Names

C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; Small-inducible cytokine C1

Protein Family

Lymphotactin

UniProt Gene Name

Xcl1

UniProt Synonym Gene Names

Lptn; Ltn; Scyc1

UniProt Entry Name

XCL1_MOUSE

NCBI Protein Information

lymphotactin

UniProt Comments for Xcl1

XCL1: Chemotactic activity for lymphocytes but not for monocytes or neutrophils. Belongs to the intercrine gamma family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; chemokine receptor binding; cytokine activity; protein homodimerization activity

Biological Process: cell-cell signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response; monocyte chemotaxis; negative regulation of interferon-gamma production; negative regulation of interleukin-2 production; negative regulation of T cell cytokine production; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of GTPase activity; positive regulation of immune response to tumor cell; positive regulation of inflammatory response; positive regulation of innate immune response; positive regulation of interleukin-10 production; positive regulation of leukocyte chemotaxis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell cytokine production; positive regulation of T cell mediated cytotoxicity; regulation of inflammatory response; release of sequestered calcium ion into cytosol; response to virus

Research Articles on Xcl1

1. T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Xcl1 elisa kit

Reference Product	PubMed Publications
Xcr1 elisa kit	>41 publications with Xcl1 and Xcr1
Ccl9 elisa kit	>3 publications with Xcl1 and Ccl9
Ccl6 elisa kit	>1 publications with Xcl1 and Ccl6

Pathways associated with Xcl1 elisa kit

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram

Diseases associated with Xcl1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>29 publications with Xcl1 and Inflammation
Disease Models, Animal Antibodies	>19 publications with Xcl1 and Disease Models, Animal
Necrosis Antibodies	>18 publications with Xcl1 and Necrosis
Neoplasms, Experimental Antibodies	>16 publications with Xcl1 and Neoplasms, Experimental
Lung Diseases Antibodies	>13 publications with Xcl1 and Lung Diseases
Hypersensitivity Antibodies	>11 publications with Xcl1 and Hypersensitivity
Cardiovascular Diseases Antibodies	>8 publications with Xcl1 and Cardiovascular Diseases
Nervous System Diseases Antibodies	>7 publications with Xcl1 and Nervous System Diseases
Multiple Myeloma Antibodies	>5 publications with Xcl1 and Multiple Myeloma
Ovarian Neoplasms Antibodies	>4 publications with Xcl1 and Ovarian Neoplasms

Organs/Tissues associated with Xcl1 elisa kit

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>19 publications with Xcl1 and Spleen
Thymus Antibodies	>8 publications with Xcl1 and Thymus
Embryonic Tissue Antibodies	>3 publications with Xcl1 and Embryonic Tissue