Serpin C1/Antithrombin-III Picokine (SERPINC1) ELISA Kit for Human

Principle of the assay: Human Serpin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Serpin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Serpin C1 amount of sample captured in plate.

Background: Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).

Small volumes of SERPINC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177593 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serpin C1/Antithrombin-III PicoKine (SERPINC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINC1. The ELISA analytical biochemical technique of the MBS177593 kit is based on SERPINC1 antibody-SERPINC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).