Mouse BMP-7 PicoKine ELISA Kit standard curve

BMP-7 Picokine (BMP7) ELISA Kit for Mouse

Name: Mouse BMP7 ELISA Kit; BMP-7 Picokine; MyBioSource
Brand: MyBioSource
SKU: MBS177606
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177606

Principle of the assay: Mouse BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard:… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

BMP-7 PicoKine (Bmp7), ELISA Kit

Full Product Name

Mouse BMP-7 PicoKine ELISA Kit

Product Gene Name

Bmp7 elisa kit

Product Synonym Names

Bone morphogenetic protein 7; BMP 7; BMP7; BMP-7; Bone morphogenetic protein 7; Eptotermin alfa; OP 1; OP1; OP1-; Osteogenic protein 1; bone morphogenetic protein 7

Species Reactivity

Mouse

Sensitivity

<10pg/ml

Specificity

Natural and recombinant mouse BMP-7

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Molecular Weight

49,199 Da

3D Structure

ModBase 3D Structure for P23359

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Assay Range

31.2pg/ml-2000pg/ml

Sample

For quantitative detection of mouse BMP-7 in bone tissue, cell culture supernates, serum and plasma((heparin, EDTA).

Immunogen

CHO, S292-H430

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BMP-7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each mouse BMP-7 standard solution and each sample be measured in duplicate.

Intra-Assay Precision

Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|185|665|1408|210|887|1699
Standard deviation|9.25|43.89|66.18|14.91|70.96|132.52
CV(%)|5.0|6.6|4.7|7.1|8.0|7.8

Sample Data

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000
O.D.|0.032|0.085|0.143|0.248|0.423|0.816|1.359|2.231

Anticoagulant

Heparin or EDTA

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of Bmp7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bmp7 purchase

MBS177606 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BMP-7 PicoKine (Bmp7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Bmp7. The ELISA analytical biochemical technique of the MBS177606 kit is based on Bmp7 antibody-Bmp7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Bmp7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Bmp7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Bmp7. It may not necessarily be applicable to this product.

NCBI GI #

31982487

NCBI GeneID

12162

NCBI Accession #

NP_031583.2

UniProt Primary Accession #

P23359

UniProt Secondary Accession #

Q91XF7

UniProt Related Accession #

P23359

GenBank Nucleotide #

NM_007557.3

NCBI Official Full Name

bone morphogenetic protein 7 preproprotein

NCBI Official Synonym Full Names

bone morphogenetic protein 7

NCBI Official Symbol

Bmp7

NCBI Official Synonym Symbols

OP1

UniProt Protein Name

Bone morphogenetic protein 7

UniProt Synonym Protein Names

Osteogenic protein 1; OP-1

Protein Family

Bone morphogenetic protein

UniProt Gene Name

Bmp7

UniProt Synonym Gene Names

Bmp-7; Op1; BMP-7; OP-1

UniProt Entry Name

BMP7_MOUSE

NCBI Protein Information

bone morphogenetic protein 7

NCBI Summary for Bmp7

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. Mutation of this gene results in skeletal, kidney, and other developmental defects. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

UniProt Comments for Bmp7

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; heparin binding; protein binding; transforming growth factor beta receptor binding

Biological Process: anatomical structure formation; axon guidance; BMP signaling pathway; branching morphogenesis of a tube; camera-type eye morphogenesis; cartilage development; cell development; cell differentiation; embryonic camera-type eye morphogenesis; embryonic limb morphogenesis; embryonic pattern specification; epithelial cell differentiation; eye development; growth; kidney development; mesenchymal cell differentiation; mesoderm formation; multicellular organismal development; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; neurite morphogenesis; odontogenesis of dentine-containing teeth; organ morphogenesis; ossification; pattern specification process; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell differentiation; positive regulation of heterotypic cell-cell adhesion; positive regulation of neuron differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of MAPKKK cascade; regulation of phosphorylation; salivary gland morphogenesis; steroid hormone mediated signaling; tube morphogenesis; ureteric bud development

Research Articles on Bmp7

1. Gdf-5 induced the expression of the alpha5 sub-unit, while Bmp-7 induced the expression of the alphaV sub-unit.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Bmp7 elisa kit

Reference Product	PubMed Publications
Bmp2 elisa kit	>339 publications with Bmp7 and Bmp2
Bmp4 elisa kit	>307 publications with Bmp7 and Bmp4
Bmpr2 elisa kit	>126 publications with Bmp7 and Bmpr2
Bmpr1a elisa kit	>46 publications with Bmp7 and Bmpr1a
Smad5 elisa kit	>45 publications with Bmp7 and Smad5
Bmpr1b elisa kit	>33 publications with Bmp7 and Bmpr1b
Acvr1 elisa kit	>27 publications with Bmp7 and Acvr1
Smad7 elisa kit	>26 publications with Bmp7 and Smad7
Acvr2a elisa kit	>6 publications with Bmp7 and Acvr2a
Nog elisa kit	>5 publications with Bmp7 and Nog

Pathways associated with Bmp7 elisa kit

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
BMP Signaling Pathway antibodies	BMP Signaling Pathway Diagram
BMP Signaling Pathway antibodies	BMP Signaling Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram

Diseases associated with Bmp7 elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>123 publications with Bmp7 and Fibrosis
Kidney Diseases Antibodies	>109 publications with Bmp7 and Kidney Diseases
Inflammation Antibodies	>61 publications with Bmp7 and Inflammation
Cardiovascular Diseases Antibodies	>57 publications with Bmp7 and Cardiovascular Diseases
Carcinoma Antibodies	>51 publications with Bmp7 and Carcinoma
Necrosis Antibodies	>39 publications with Bmp7 and Necrosis
Renal Insufficiency Antibodies	>39 publications with Bmp7 and Renal Insufficiency
Bone Resorption Antibodies	>37 publications with Bmp7 and Bone Resorption
Hypertension Antibodies	>37 publications with Bmp7 and Hypertension
Lung Diseases Antibodies	>28 publications with Bmp7 and Lung Diseases

Organs/Tissues associated with Bmp7 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>180 publications with Bmp7 and Embryonic Tissue
Brain Antibodies	>120 publications with Bmp7 and Brain
Bone Marrow Antibodies	>101 publications with Bmp7 and Bone Marrow
Eye Antibodies	>78 publications with Bmp7 and Eye
Lung Antibodies	>45 publications with Bmp7 and Lung
Spinal Cord Antibodies	>31 publications with Bmp7 and Spinal Cord
Stomach Antibodies	>8 publications with Bmp7 and Stomach
Pancreas Antibodies	>8 publications with Bmp7 and Pancreas
Adrenal Gland Antibodies	>6 publications with Bmp7 and Adrenal Gland
Dorsal Root Ganglion Antibodies	>3 publications with Bmp7 and Dorsal Root Ganglion