DCR3/TNFRSF6B Picokine (TNFRSF6B) ELISA Kit for Human

Principle of the Assay: The human DCR3 ELISA Kit was based on standard sandwich enzyme-lirked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DCR3 has been precoate-J onto 96-well plates. Standards (Expression system for standard: sf21. Immunogen sequence: V24 - H300) and test samples are added to the wells, a biotinylated detection polyc!onal antibody from goat specific for DCR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCR3 amount of sample captured in plate.

Background/Introduction: Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL 1A. Overexpression of this gene has be':!n noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.

Small volumes of TNFRSF6B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177623 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DCR3/TNFRSF6B PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNFRSF6B. The ELISA analytical biochemical technique of the MBS177623 kit is based on TNFRSF6B antibody-TNFRSF6B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNFRSF6B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNFRSF6B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).