Rat E-Cadherin PicoKine ELISA Kit standard curve

E-Cadherin Picokine (CDH1) ELISA Kit for Rat

Name: Rat CDH1 ELISA Kit; E-Cadherin Picokine; MyBioSource
Brand: MyBioSource
SKU: MBS177628
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177628

Principle of the assay: Rat E-Cadherin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for E-Cadherin has been precoated onto 96-well plates. Standards(Expression system for… More details

$635 / 96-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

E-Cadherin PicoKine (Cdh1), ELISA Kit

Full Product Name

Rat E-Cadherin PicoKine ELISA Kit

Product Gene Name

Cdh1 elisa kit

Product Synonym Names

Cadherin-1; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; cadherin 1, type 1, E-cadherin (epithelial)

Species Reactivity

Rat

Sensitivity

<15pg/ml

Specificity

Natural and recombinant rat E-Cadherin

Preparation and Storage

Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Molecular Weight

98,715 Da

3D Structure

ModBase 3D Structure for Q9R0T4

Production Time

Due to severe delay and raw materials shortage, production time may takes between 5-8 weeks.

Assay Range

156pg/ml-10000pg/ml

Sample

For quantitative detection of rat E-Cadherin in cell culture supernates, serum and plasma(heparin).

Immunogen

NSO, Q24-S704

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat E-Cadherin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each rat E-Cadherin standard solution and each sample be measured in duplicate.

Intra-Assay Precision

Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1571|4158|7193|1690|4873|8056
Standard deviation|75.41|228.69|431.58|119.99|302.13|459.19
CV(%)|4.8|5.5|6.0|7.1|6.2|5.7

Sample Data

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000
O.D.|0.097|0.100|0.163|0.252|0.482|0.930|1.654|2.438

Anticoagulant

Heparin

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of Cdh1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cdh1 purchase

MBS177628 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the E-Cadherin PicoKine (Cdh1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cdh1. The ELISA analytical biochemical technique of the MBS177628 kit is based on Cdh1 antibody-Cdh1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cdh1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cdh1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Cdh1. It may not necessarily be applicable to this product.

NCBI GI #

13786164

NCBI GeneID

83502

NCBI Accession #

NP_112624.1

UniProt Primary Accession #

Q9R0T4

UniProt Secondary Accession #

O35794; Q9JIV9

UniProt Related Accession #

Q9R0T4

GenBank Nucleotide #

NM_031334.1

NCBI Official Full Name

cadherin-1

NCBI Official Synonym Full Names

cadherin 1

NCBI Official Symbol

Cdh1

UniProt Protein Name

Cadherin-1

UniProt Synonym Protein Names

Epithelial cadherin; E-cadherin

Protein Family

Anaphase-promoting complex

UniProt Gene Name

Cdh1

UniProt Synonym Gene Names

E-cadherin

UniProt Entry Name

CADH1_RAT

NCBI Protein Information

cadherin-1

NCBI Summary for Cdh1

cell-cell adhesion molecule; may play a role in axonal growth and synase formation [RGD, Feb 2006]

UniProt Comments for Cdh1

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Ubiquitin ligase; Membrane protein, integral

Cellular Component: actin cytoskeleton; adherens junction; apical junction complex; apical part of cell; axon; basolateral plasma membrane; catenin complex; cell junction; cell surface; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; endosome; flotillin complex; focal adhesion; integral to membrane; intercellular junction; internal side of plasma membrane; lamellipodium; lateral loop; lateral plasma membrane; nerve terminal; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: ankyrin binding; beta-catenin binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; glycoprotein binding; GTPase activating protein binding; protein binding; protein domain specific binding; protein phosphatase binding

Biological Process: calcium-dependent cell-cell adhesion; cell-cell adhesion; decidualization; embryo implantation; homophilic cell adhesion; in utero embryonic development; negative regulation of cell-cell adhesion; negative regulation of epithelial cell proliferation; neurite development; pituitary gland development; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent; protein homooligomerization; protein metabolic process; regulation of caspase activity; regulation of protein localization; regulation of water loss via skin; response to drug; response to organic substance; response to toxin; sensory perception of sound; synaptogenesis; trophectodermal cell differentiation

Research Articles on Cdh1

1. Results suggest a regulatory function of E-cadherin that modulates Nrg1 signaling and promotes Schwann cell myelin formation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Cdh1 elisa kit

Reference Product	PubMed Publications
Ctnnb1 elisa kit	>234 publications with Cdh1 and Ctnnb1
Egfr elisa kit	>65 publications with Cdh1 and Egfr
Ctnna1 elisa kit	>19 publications with Cdh1 and Ctnna1
Casp3 elisa kit	>10 publications with Cdh1 and Casp3
Mmp7 elisa kit	>6 publications with Cdh1 and Mmp7
Jup elisa kit	>5 publications with Cdh1 and Jup
Cbll1 elisa kit	>2 publications with Cdh1 and Cbll1
Klrg1 elisa kit	>1 publications with Cdh1 and Klrg1
Ctnnd1 elisa kit	>1 publications with Cdh1 and Ctnnd1

Pathways associated with Cdh1 elisa kit

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junctions Interactions Pathway antibodies	Adherens Junctions Interactions Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptotic Cleavage Of Cell Adhesion Proteins Pathway antibodies	Apoptotic Cleavage Of Cell Adhesion Proteins Pathway Diagram
Apoptotic Cleavage Of Cellular Proteins Pathway antibodies	Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
Apoptotic Execution Phase Pathway antibodies	Apoptotic Execution Phase Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram

Diseases associated with Cdh1 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>649 publications with Cdh1 and Carcinoma
Stomach Neoplasms Antibodies	>369 publications with Cdh1 and Stomach Neoplasms
Breast Neoplasms Antibodies	>319 publications with Cdh1 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>231 publications with Cdh1 and Neoplasm Metastasis
Neoplasm Invasiveness Antibodies	>207 publications with Cdh1 and Neoplasm Invasiveness
Disease Progression Antibodies	>147 publications with Cdh1 and Disease Progression
Colorectal Neoplasms Antibodies	>134 publications with Cdh1 and Colorectal Neoplasms
Lung Neoplasms Antibodies	>99 publications with Cdh1 and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>92 publications with Cdh1 and Carcinoma, Squamous Cell
Liver Neoplasms Antibodies	>83 publications with Cdh1 and Liver Neoplasms

Organs/Tissues associated with Cdh1 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>141 publications with Cdh1 and Lung
Liver Antibodies	>103 publications with Cdh1 and Liver
Brain Antibodies	>76 publications with Cdh1 and Brain
Prostate Antibodies	>70 publications with Cdh1 and Prostate
Colon Antibodies	>69 publications with Cdh1 and Colon
Embryonic Tissue Antibodies	>54 publications with Cdh1 and Embryonic Tissue
Kidney Antibodies	>53 publications with Cdh1 and Kidney
Ovary Antibodies	>23 publications with Cdh1 and Ovary
Pancreas Antibodies	>19 publications with Cdh1 and Pancreas
Placenta Antibodies	>15 publications with Cdh1 and Placenta