Tenascin-C/TNC Picokine (TNC) ELISA Kit for Human

Principle of the Assay: The human Tenascin-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tenascin-C has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: G23-P625) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Tenascin-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution The density of yellow is proportional to the human Tenascin-C amount of sample captured in plate.

Background: Tenascln C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

Small volumes of TNC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS177649 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tenascin-C/TNC PicoKine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TNC. The ELISA analytical biochemical technique of the MBS177649 kit is based on TNC antibody-TNC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TNC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TNC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).