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FASN Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177671

Description: Rabbit IgG polyclonal antibody for Fatty acid synthase(FASN) detection. Tested with WB, IHC-P in Human.Background: Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded… More details

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Product Overview

Online Datasheet in PDF
Product Name
FASN, Polyclonal Antibody
Full Product Name
Anti-FASN Antibody
Product Gene Name
anti-FASN antibody
Product Synonym Names
Fatty acid synthase; [Acyl-carrier-protein] S acetyltransferase; [Acyl-carrier-protein] S malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; FAS; FAS_HUMAN; FASN; Fatty acid synthase; MGC14367; MGC15706; OA 519; Oleoyl-[acyl-carrier-protein] hydrolase; SDR27X1; Short chain dehydrogenase/reductase family 27X member 1; fatty acid synthase
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
273,427 Da
OMIM
600212
3D Structure
ModBase 3D Structure for P49327
Immunogen
E Coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-FASN antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-FASN antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-FASN antibody

Western blot analysis of FASN expression in HEPG2 whole cell lysates (lane 1), U87 whole cell lysates (lane 2) and RAJI whole cell lysates (lane 3). FASN at 273KD was detected using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.
Anti-FASN Polyclonal Antibody – Cat# MBS177671

Immunohistochemistry (IHC) of anti-FASN antibody

FASN was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- FASN Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.
Anti-FASN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177671

Immunohistochemistry (IHC) of anti-FASN antibody

Figure 3. IHC analysis of FASN using anti-FASN antibody (MBS177671).FASN was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FASN Antibody (MBS177671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-FASN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177671

Immunohistochemistry (IHC) of anti-FASN antibody

Figure 4. IHC analysis of FASN using anti-FASN antibody (MBS177671).FASN was detected in immunocytochemical section of MCF-7 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FASN Antibody (MBS177671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-FASN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177671

Product References and Citations for anti-FASN antibody

1. "Entrez Gene: FASN fatty acid synthase". 2. Jayakumar A, Tai MH, Huang WY, al-Feel W, Hsu M, Abu-Elheiga L, Chirala SS, Wakil SJ (Oct 1995). "Human fatty acid synthase: properties and molecular cloning". Proc Natl Acad Sci U S A 92 (19): 8695-9. 3. Smith S, Witkowski A, Joshi AK (July 2003). "Structural and functional organization of the animal fatty acid synthase". Prog. Lipid Res. 42 (4): 289-317.

Other Notes

Small volumes of anti-FASN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FASN purchase

MBS177671 is an antibody targeting FASN. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for FASN. It may not necessarily be applicable to this product.

NCBI GI #
41872631
NCBI GeneID
2194
NCBI Accession #
NP_004095.4
UniProt Primary Accession #
P49327
UniProt Secondary Accession #
Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
UniProt Related Accession #
P49327
GenBank Nucleotide #
NM_004104.4
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
UniProt Protein Name
Fatty acid synthase
Protein Family
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
UniProt Entry Name
FAS_HUMAN
NCBI Protein Information
fatty acid synthase

NCBI Summary for FASN

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
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UniProt Comments for FASN

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: EC 1.3.1.39; EC 2.3.1.41; EC 1.1.1.100; Lipid Metabolism - fatty acid biosynthesis; EC 2.3.1.38; EC 3.1.2.14; Hydrolase; EC 2.3.1.39; EC 4.2.1.59; Acetyltransferase; Lyase; EC 2.3.1.85; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; cytosol; glycogen granule; Golgi apparatus; melanosome; membrane; mitochondrion; plasma membrane

Molecular Function: 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-malonyltransferase activity; drug binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; phosphopantetheine binding; protein binding; protein homodimerization activity

Biological Process: acetyl-CoA metabolic process; fatty acid biosynthetic process; fatty acid metabolic process; mammary gland development; osteoblast differentiation; pantothenate metabolic process; positive regulation of cellular metabolic process
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Research Articles on FASN

1. In the present study it was determined whether three key enzymes for glycolysis, glutaminolysis and de novo synthesis of FAs, hexokinase-2, glutaminase and fatty acid synthase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FASN antibody

 Reference Product  PubMed Publications
 SREBF1 antibody  >153 publications with FASN and SREBF1
 ACACA antibody  >101 publications with FASN and ACACA
 LEP antibody  >11 publications with FASN and LEP
 ACACB antibody  >7 publications with FASN and ACACB
 INS antibody  >2 publications with FASN and INS
 OLAH antibody  >1 publications with FASN and OLAH

Pathways associated with anti-FASN antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram

Diseases associated with anti-FASN antibody

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >195 publications with FASN and Fatty Liver
 Obesity Antibodies  >138 publications with FASN and Obesity
 Liver Diseases Antibodies  >85 publications with FASN and Liver Diseases
 Insulin Resistance Antibodies  >69 publications with FASN and Insulin Resistance
 Breast Neoplasms Antibodies  >62 publications with FASN and Breast Neoplasms
 Inflammation Antibodies  >56 publications with FASN and Inflammation
 Weight Gain Antibodies  >32 publications with FASN and Weight Gain
 Carcinoma, Hepatocellular Antibodies  >27 publications with FASN and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >27 publications with FASN and Liver Neoplasms
 Weight Loss Antibodies  >27 publications with FASN and Weight Loss

Organs/Tissues associated with anti-FASN antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >228 publications with FASN and Liver
 Adipose Tissue Antibodies  >154 publications with FASN and Adipose Tissue
 Blood Antibodies  >142 publications with FASN and Blood
 Connective Tissue Antibodies  >100 publications with FASN and Connective Tissue
 Muscle Antibodies  >72 publications with FASN and Muscle
 Prostate Antibodies  >58 publications with FASN and Prostate
 Mammary Gland Antibodies  >43 publications with FASN and Mammary Gland
 Brain Antibodies  >28 publications with FASN and Brain
 Lung Antibodies  >27 publications with FASN and Lung
 Kidney Antibodies  >20 publications with FASN and Kidney
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