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PKM2 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177672

Description: Rabbit IgG polyclonal antibody for Pyruvate kinase isozymes M1/M2(PKM) detection. Tested with WB, IHC-P in Human.Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The… More details

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Product Overview

Online Datasheet in PDF
Product Name
PKM2, Polyclonal Antibody
Full Product Name
Anti-PKM2 Antibody
Product Gene Name
anti-PKM2 antibody
Product Synonym Names
Pyruvate kinase isozymes M1/M2; CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; MGC3932; OIP 3; OIP-3; Oip3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK Muscle type; PK, muscle type; PK2; Pk3; PKM; PKM2; PYKM; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase, muscle 2; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK; pyruvate kinase, muscle
Clonality
Polyclonal
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
56,273 Da
OMIM
179050
3D Structure
ModBase 3D Structure for P14618
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PKM2 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-PKM2 antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-PKM2 antibody

Anti- PKM2 Picoband antibody, MBS177672, Western blotting
All lanes: Anti PKM2 (MBS177672) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: MM453 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 58KD
Anti-PKM2 Polyclonal Antibody – Cat# MBS177672

Immunohistochemistry (IHC) of anti-PKM2 antibody

Anti- PKM2 Picoband antibody, MBS177672, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-PKM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177672

Product References and Citations for anti-PKM2 antibody

1. Ashizawa, K., Willingham, M. C., Liang, C.-M., Cheng, S. In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M-2 by glucose is mediated via fructose 1, 6-bisphosphate. 2. J. Biol. Chem. 266: 16842-16846, 1991. 2. Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., Cantley, L. C. Pyruvate kinase M2 is a phosphotyrosine-binding protein. 3. Nature 452: 181-186, 2008. 3. Popescu, N. C., Cheng, S. Y. Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M(2). Somat. Cell Molec. Genet. 16: 593-598, 1990.

Other Notes

Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS177672 is an antibody targeting PKM2. Reactive in Human.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #
332164775
NCBI GeneID
5315
NCBI Accession #
NP_001193725.1
UniProt Primary Accession #
P14618
UniProt Secondary Accession #
P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
UniProt Related Accession #
P14618
GenBank Nucleotide #
NM_001206796.2
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
NCBI Protein Information
pyruvate kinase PKM

NCBI Summary for PKM2

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
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UniProt Comments for PKM2

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Research Articles on PKM2

1. The PARP14-JNK1-PKM2 regulatory axis is an important determinant for the Warburg effect in tumour cells and provides a mechanistic link between apoptosis and metabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PKM2 antibody

 Reference Product  PubMed Publications
 HIF1A antibody  >9 publications with PKM2 and HIF1A
 ENO1 antibody  >6 publications with PKM2 and ENO1
 LDHB antibody  >3 publications with PKM2 and LDHB
 GPI antibody  >3 publications with PKM2 and GPI
 ALDOA antibody  >1 publications with PKM2 and ALDOA
 ENO2 antibody  >1 publications with PKM2 and ENO2
 PGK1 antibody  >1 publications with PKM2 and PGK1

Pathways associated with anti-PKM2 antibody

 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram

Diseases associated with anti-PKM2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >16 publications with PKM2 and Nervous System Diseases
 Inflammation Antibodies  >9 publications with PKM2 and Inflammation
 Carcinoma Antibodies  >9 publications with PKM2 and Carcinoma
 Fibrosis Antibodies  >8 publications with PKM2 and Fibrosis
 Lung Diseases Antibodies  >8 publications with PKM2 and Lung Diseases
 Cardiovascular Diseases Antibodies  >8 publications with PKM2 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >7 publications with PKM2 and Cell Transformation, Neoplastic
 Necrosis Antibodies  >5 publications with PKM2 and Necrosis
 Liver Diseases Antibodies  >5 publications with PKM2 and Liver Diseases
 Disease Models, Animal Antibodies  >4 publications with PKM2 and Disease Models, Animal

Organs/Tissues associated with anti-PKM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >63 publications with PKM2 and Brain
 Blood Antibodies  >43 publications with PKM2 and Blood
 Muscle Antibodies  >33 publications with PKM2 and Muscle
 Liver Antibodies  >18 publications with PKM2 and Liver
 Kidney Antibodies  >16 publications with PKM2 and Kidney
 Thyroid Antibodies  >12 publications with PKM2 and Thyroid
 Nerve Antibodies  >11 publications with PKM2 and Nerve
 Vascular Antibodies  >11 publications with PKM2 and Vascular
 Heart Antibodies  >11 publications with PKM2 and Heart
 Lung Antibodies  >7 publications with PKM2 and Lung
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