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CD22 Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177673

Description: Rabbit IgG polyclonal antibody for B-cell receptor CD22(CD22) detection. Tested with WB, IHC-P in Human.Background: CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CD22, Polyclonal Antibody
Full Product Name
Anti-CD22 Antibody
Product Gene Name
anti-CD22 antibody
Product Synonym Names
B-cell receptor CD22; B cell receptor CD22 precursor; B lymphocyte cell adhesion molecule; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL CAM; BL-CAM; BLCAM; CD 22; CD22; CD22 antigen; CD22 molecule; CD22 protein; CD22_HUMAN; Lectin 2; Leu14; Lyb8; MGC130020; sialic acid binding Ig like lectin 2; Sialic acid binding immunoglobulin like lectin 2; Sialic acid-binding Ig-like lectin 2; SIGLEC 2; Siglec-2; SIGLEC2; T cell surface antigen Leu 14; T-cell surface antigen Leu-14; CD22 molecule
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
OMIM
107266
3D Structure
ModBase 3D Structure for P20273
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD22 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-CD22 antibody
WB: 0.1-0.5ug/ml
IHC: 0.5-1ug/ml
FC: 1-3ug/1x106

Tested Species: In-house tested species with positive test results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-CD22 antibody

Anti- CD22 Picoband antibody, MBS177673, Western blotting
All lanes: Anti CD22 (MBS177673) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: 22RV1 Whole Cell Lysate at 40ug
Predicted bind size: 95KD
Observed bind size: 95KD
Anti-CD22 Polyclonal Antibody – Cat# MBS177673

Immunohistochemistry (IHC) of anti-CD22 antibody

Anti- CD22 Picoband antibody, MBS177673, IHC(P)
IHC(P): Human Tonsil Tissue
Anti-CD22 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177673

Flow Cytometry (FC/FACS) of anti-CD22 antibody

Figure 3. Flow Cytometry analysis of Raji cells using anti-CD22 antibody (MBS177673).
Overlay histogram showing Raji cells stained with MBS177673 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (MBS177673,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-CD22 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS177673

Product References and Citations for anti-CD22 antibody

1. John, B.; Herrin, B. R.; Raman, C.; Wang, Y.; Bobbitt, K. R.; Brody, B. A.; Justement, L. B. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interaction. J. Immun. 170: 3534-3543, 2003. 2. O'Keefe, T. L.; Williams, G. T.; Davies, S. L.; Neuberger, M. S. Hyperresponsive B cells in CD22-deficient mice. Science 274: 798-801, 1996. 3. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H. Genomic structure and chromosomal mapping of the human CD22 gene. J. Immun. 150: 5013-5024, 1993.

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD22 purchase

MBS177673 is an antibody targeting CD22. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #
297374825
NCBI GeneID
933
NCBI Accession #
NP_001172028.1
UniProt Primary Accession #
P20273
UniProt Secondary Accession #
O95699; O95701; O95702; O95703; Q01665; Q32M46; Q92872; Q92873; Q9UQA6; F5GYU4; F5H7U3
UniProt Related Accession #
P20273
GenBank Nucleotide #
NM_001185099.1
NCBI Official Full Name
B-cell receptor CD22 isoform 2
NCBI Official Synonym Full Names
CD22 molecule
NCBI Official Symbol
CD22
NCBI Official Synonym Symbols
SIGLEC2; SIGLEC-2
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22
Protein Family
B-cell receptor
UniProt Gene Name
CD22
UniProt Synonym Gene Names
SIGLEC2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_HUMAN
NCBI Protein Information
B-cell receptor CD22

UniProt Comments for CD22

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbohydrate binding; protein binding

Biological Process: cell adhesion
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Research Articles on CD22

1. results demonstrate that loss of high affinity CD22 ligands on GC B-cells occurs in both mice and humans through alternative mechanisms, unmasking CD22 relative to naive and memory B-cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD22 antibody

 Reference Product  PubMed Publications
 CD19 antibody  >660 publications with CD22 and CD19
 BCR antibody  >155 publications with CD22 and BCR
 CD79B antibody  >80 publications with CD22 and CD79B
 CD79A antibody  >73 publications with CD22 and CD79A
 LYN antibody  >48 publications with CD22 and LYN
 SYK antibody  >33 publications with CD22 and SYK
 GRB2 antibody  >10 publications with CD22 and GRB2
 PTPRC antibody  >4 publications with CD22 and PTPRC

Pathways associated with anti-CD22 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 BCR Signaling Pathway antibodies  BCR Signaling Pathway Diagram
 CD22 Mediated BCR Regulation Pathway antibodies  CD22 Mediated BCR Regulation Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram

Diseases associated with anti-CD22 antibody

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >1001 publications with CD22 and Immune System Diseases
 Chromosome Aberrations Antibodies  >77 publications with CD22 and Chromosome Aberrations
 Inflammation Antibodies  >62 publications with CD22 and Inflammation
 Respiratory Tract Diseases Antibodies  >50 publications with CD22 and Respiratory Tract Diseases
 Necrosis Antibodies  >50 publications with CD22 and Necrosis
 Disease Models, Animal Antibodies  >49 publications with CD22 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >24 publications with CD22 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >23 publications with CD22 and Kidney Diseases
 Lung Diseases Antibodies  >23 publications with CD22 and Lung Diseases
 Liver Diseases Antibodies  >20 publications with CD22 and Liver Diseases

Organs/Tissues associated with anti-CD22 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1087 publications with CD22 and Blood
 Bone Marrow Antibodies  >301 publications with CD22 and Bone Marrow
 Spleen Antibodies  >125 publications with CD22 and Spleen
 Lymph Node Antibodies  >114 publications with CD22 and Lymph Node
 Lymph Antibodies  >102 publications with CD22 and Lymph
 Skin Antibodies  >52 publications with CD22 and Skin
 Lung Antibodies  >34 publications with CD22 and Lung
 Kidney Antibodies  >32 publications with CD22 and Kidney
 Tonsil Antibodies  >31 publications with CD22 and Tonsil
 Thymus Antibodies  >30 publications with CD22 and Thymus
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