Anti-SMURF1 Polyclonal Antibody – Cat# MBS177689

Western blot analysis of SMURF1 expression in rat brain extract (lane 1) and MCF-7 whole cell lysates (lane 2). SMURF1 at 86KD was detected using rabbit anti- SMURF1 Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method.

SMURF1 Antibody – Polyclonal

Name: SMURF1 Antibody; Polyclonal; Human/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS177689
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177689

Description: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase SMURF1(SMURF1) detection. Tested with WB in Human;Rat.Background: E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1.… More details

$210 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SMURF1, Polyclonal Antibody

Full Product Name

Anti-SMURF1 Antibody

Product Gene Name

anti-SMURF1 antibody

Product Synonym Names

E3 ubiquitin-protein ligase SMURF1; E3 ubiquitin-protein ligase SMURF1; hSMURF1; KIAA1625; Smad specific E3 ubiquitin ligase 1; SMAD specific E3 ubiquitin protein ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMUF1_HUMAN; SMURF 1; smurf1; SMAD specific E3 ubiquitin protein ligase 1

Clonality

Polyclonal

Species Reactivity

Human, Rat

Form/Format

Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purity/Purification

Immunogen Affinity Purified

Concentration

1 mg/ml (lot specific)

Preparation and Storage

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

83,440 Da

OMIM

605568

3D Structure

ModBase 3D Structure for Q9HCE7

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-SMURF1 antibody

Western Blot (WB)

Application Notes for anti-SMURF1 antibody

Western Blot Concentration: 0.1-0.5ug/ml

Product References and Citations for anti-SMURF1 antibody

1. "Entrez Gene: SMURF1 SMAD specific E3 ubiquitin protein ligase 1". 2. Yamaguchi K, Ohara O, Ando A, Nagase T (Apr 2008). "Smurf1 directly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway". Biol. Chem. 389 (4): 405-13. 3. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH (Sep 1999). "A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation". Nature 400 (6745): 687-93.

Other Notes

Small volumes of anti-SMURF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMURF1 purchase

MBS177689 is an antibody targeting SMURF1. Reactive in Human, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for SMURF1. It may not necessarily be applicable to this product.

NCBI GI #

315434204

NCBI GeneID

57154

NCBI Accession #

NP_001186776.1

UniProt Primary Accession #

Q9HCE7

UniProt Secondary Accession #

O75853; Q547Q3; Q9UJT8; A4D279; B7ZMB6; B9EGV3

UniProt Related Accession #

Q9HCE7

GenBank Nucleotide #

NM_001199847.1

NCBI Official Full Name

E3 ubiquitin-protein ligase SMURF1 isoform 3

NCBI Official Synonym Full Names

SMAD specific E3 ubiquitin protein ligase 1

NCBI Official Symbol

SMURF1

UniProt Protein Name

E3 ubiquitin-protein ligase SMURF1

UniProt Synonym Protein Names

SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

SMURF1

UniProt Synonym Gene Names

KIAA1625; hSMURF1

UniProt Entry Name

SMUF1_HUMAN

NCBI Protein Information

E3 ubiquitin-protein ligase SMURF1

NCBI Summary for SMURF1

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]

UniProt Comments for SMURF1

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: axon; cell soma; cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane

Molecular Function: activin binding; ligase activity; phospholipid binding; protein binding; ubiquitin-protein ligase activity

Biological Process: BMP signaling pathway; cell differentiation; ectoderm development; negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of defense response to virus by host; proteasomal ubiquitin-dependent protein catabolic process; protein export from nucleus; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway

Research Articles on SMURF1

1. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMURF1 antibody

Reference Product	PubMed Publications
SMAD1 antibody	>42 publications with SMURF1 and SMAD1
RHOA antibody	>40 publications with SMURF1 and RHOA
SMAD7 antibody	>34 publications with SMURF1 and SMAD7
SMAD2 antibody	>24 publications with SMURF1 and SMAD2
SMAD4 antibody	>18 publications with SMURF1 and SMAD4
SMAD5 antibody	>18 publications with SMURF1 and SMAD5
SMAD3 antibody	>17 publications with SMURF1 and SMAD3
SMAD6 antibody	>17 publications with SMURF1 and SMAD6

Pathways associated with anti-SMURF1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies	Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
Asymmetric Localization Of PCP Proteins Pathway antibodies	Asymmetric Localization Of PCP Proteins Pathway Diagram
BMP Receptor Signaling Pathway antibodies	BMP Receptor Signaling Pathway Diagram
BMP Signalling And Regulation Pathway antibodies	BMP Signalling And Regulation Pathway Diagram
Beta-catenin Independent WNT Signaling Pathway antibodies	Beta-catenin Independent WNT Signaling Pathway Diagram
Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies	Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
Downregulation Of TGF-beta Receptor Signaling Pathway antibodies	Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram

Diseases associated with anti-SMURF1 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>13 publications with SMURF1 and Necrosis
Inflammation Antibodies	>9 publications with SMURF1 and Inflammation
Fibrosis Antibodies	>8 publications with SMURF1 and Fibrosis
Breast Neoplasms Antibodies	>8 publications with SMURF1 and Breast Neoplasms
Liver Diseases Antibodies	>7 publications with SMURF1 and Liver Diseases
Liver Neoplasms Antibodies	>7 publications with SMURF1 and Liver Neoplasms
Neoplasm Metastasis Antibodies	>5 publications with SMURF1 and Neoplasm Metastasis
Kidney Diseases Antibodies	>5 publications with SMURF1 and Kidney Diseases
Cardiovascular Diseases Antibodies	>5 publications with SMURF1 and Cardiovascular Diseases
Adenocarcinoma Antibodies	>5 publications with SMURF1 and Adenocarcinoma

Organs/Tissues associated with anti-SMURF1 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>89 publications with SMURF1 and Bone
Muscle Antibodies	>13 publications with SMURF1 and Muscle
Connective Tissue Antibodies	>12 publications with SMURF1 and Connective Tissue
Embryonic Tissue Antibodies	>11 publications with SMURF1 and Embryonic Tissue
Kidney Antibodies	>10 publications with SMURF1 and Kidney
Heart Antibodies	>9 publications with SMURF1 and Heart
Liver Antibodies	>9 publications with SMURF1 and Liver
Bone Marrow Antibodies	>8 publications with SMURF1 and Bone Marrow
Brain Antibodies	>8 publications with SMURF1 and Brain
Blood Antibodies	>6 publications with SMURF1 and Blood