• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ARSA Antibody – Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS177695

Description: Rabbit IgG polyclonal antibody for Arylsulfatase A(ARSA) detection. Tested with WB, IHC-P in Human;Mouse;Rat.Background: Arylsulfatase A (ARSA) is an enzyme that breaks down sulfatides, namely cerebroside 3-sulfate intocerebroside and sulfate. In… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARSA, Polyclonal Antibody
Full Product Name
Anti-ARSA Antibody
Product Gene Name
anti-ARSA antibody
Product Synonym Names
Arylsulfatase A; arsA; ARSA_HUMAN; arylsulfatase A; Arylsulfatase A component C; As 2; AS A; As2; ASA; AW212749; C230037L18Rik; Cerebroside-sulfatase; metachromatic leucodystrophy; MGC125207; MLD; OTTHUMP00000196546; OTTHUMP00000196548; TISP73; arylsulfatase A
Clonality
Polyclonal
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purity/Purification
Immunogen Affinity Purified
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
44,881 Da
OMIM
250100
3D Structure
ModBase 3D Structure for P15289
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA (454-482aa QALKQLQLLKAQLDAAVTFGPSQVARGED), different from the related mouse sequence by six amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-ARSA antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-ARSA antibody
Western Blot Concentration: 0.1-0.5ug/ml
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml

Western Blot (WB) of anti-ARSA antibody

Figure 1. Western blot analysis of ARSA using anti-ARSA antibody (MBS177695).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Testis Tissue Lysate,
Lane 2: Rat Pancreas Tissue Lysate,
Lane 3: Rat Skeletal Muscle Tissue Lysate,
Lane 4: Mouse Kidney Tissue Lysate,
Lane 5: MCF-7 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARSA at approximately 54KD. The expected band size for ARSA is at 54KD.
Anti-ARSA Polyclonal Antibody – Cat# MBS177695

Immunohistochemistry (IHC) of anti-ARSA antibody

Figure 2. IHC analysis of ARSA using anti-ARSA antibody (MBS177695).
ARSA was detected in paraffin-embedded section of Rat Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARSA Antibody (MBS177695) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-ARSA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177695

Immunohistochemistry (IHC) of anti-ARSA antibody

Figure 3. IHC analysis of ARSA using anti-ARSA antibody (MBS177695).
ARSA was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARSA Antibody (MBS177695) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Anti-ARSA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS177695

Flow Cytometry (FC/FACS) of anti-ARSA antibody

Figure 4. Flow Cytometry analysis of Hela cells using anti-ARSA antibody (MBS177695).
Overlay histogram showing Hela cells stained with MBS177695 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (MBS177695,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-ARSA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS177695

Flow Cytometry (FC/FACS) of anti-ARSA antibody

Figure 5. Flow Cytometry analysis of PC-3 cells using anti-ARSA antibody (MBS177695).
Overlay histogram showing PC-3 cells stained with MBS177695 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (MBS177695,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-ARSA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS177695

Product References and Citations for anti-ARSA antibody

1. Fujii T; Kobayashi T; Honke K et al. (1992). "Proteolytic processing of human lysosomal arylsulfatase A". Biochim. Biophys. Acta 1122 (1): 93-8. 2. Kondo R; Wakamatsu N; Yoshino H et al. (1991)."Identification of a mutation in the arylsulfatase A gene of a patient with adult-type metachromatic leukodystrophy". Am. J. Hum. Genet. 48 (5): 971-8. 3. Matzner U, Herbst E, Hedayati KK, Lüllmann-Rauch R, Wessig C, Schröder S, Eistrup C, Möller C, Fogh J, Gieselmann V (May 2005). "Enzyme replacement improves nervous system pathology and function in a mouse model for metachromatic leukodystrophy". Hum. Mol. Genet. 14 (9): 1139-52.

Other Notes

Small volumes of anti-ARSA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARSA purchase

MBS177695 is an antibody targeting ARSA. Reactive in Human, Mouse, Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for ARSA. It may not necessarily be applicable to this product.

NCBI GI #
313569791
NCBI GeneID
410
NCBI Accession #
NP_000478.3
UniProt Primary Accession #
P15289
UniProt Secondary Accession #
Q6ICI5; Q96CJ0; B2RCA6; B7XD04; F8WCC8
UniProt Related Accession #
P15289
GenBank Nucleotide #
NM_000487.5
NCBI Official Full Name
arylsulfatase A isoform a
NCBI Official Synonym Full Names
arylsulfatase A
NCBI Official Symbol
ARSA
NCBI Official Synonym Symbols
MLD
UniProt Protein Name
Arylsulfatase A
UniProt Synonym Protein Names
Cerebroside-sulfatase
Protein Family
Arsenical pump-driving ATPase
UniProt Gene Name
ARSA
UniProt Synonym Gene Names
ASA
UniProt Entry Name
ARSA_HUMAN
NCBI Protein Information
arylsulfatase A

NCBI Summary for ARSA

The protein encoded by this gene hydrolyzes cerebroside sulfate to cerebroside and sulfate. Defects in this gene lead to metachromatic leucodystrophy (MLD), a progressive demyelination disease which results in a variety of neurological symptoms and ultimately death. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2010]
Show more

UniProt Comments for ARSA

ARSA: Hydrolyzes cerebroside sulfate. Defects in ARSA are a cause of leukodystrophy metachromatic (MLD). MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late- infantile, juvenile and adult. Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD). A clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Arylsulfatase A activity is impaired in multiple sulfatase deficiency due to mutations in SUMF1. SUMF1 mutations result in defective post-translational modification of ARSA at residue Cys- 69 that is not converted to 3-oxoalanine. Belongs to the sulfatase family.

Protein type: EC 3.1.6.8; Hydrolase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: endoplasmic reticulum lumen; lysosomal lumen; lysosome

Molecular Function: arylsulfatase activity; calcium ion binding; cerebroside-sulfatase activity; protein binding; sulfuric ester hydrolase activity

Biological Process: glycosphingolipid metabolic process; post-translational protein modification

Disease: Metachromatic Leukodystrophy
Show more

Research Articles on ARSA

1. We report three families with Arylsulphatase A partial deficit in which we can find a high recurrence of parkinsonism among the siblings.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARSA antibody

 Products by Pathway  Pathway Diagram
 Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies  Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
 Glycosphingolipid Metabolism Pathway antibodies  Glycosphingolipid Metabolism Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram

Diseases associated with anti-ARSA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >125 publications with ARSA and Nervous System Diseases
 Leukodystrophy, Metachromatic Antibodies  >107 publications with ARSA and Leukodystrophy, Metachromatic
 Heart Diseases Antibodies  >20 publications with ARSA and Heart Diseases
 Liver Diseases Antibodies  >5 publications with ARSA and Liver Diseases
 Poisoning Antibodies  >3 publications with ARSA and Poisoning
 Lung Neoplasms Antibodies  >2 publications with ARSA and Lung Neoplasms
 Infertility, Male Antibodies  >2 publications with ARSA and Infertility, Male
 Weight Loss Antibodies  >2 publications with ARSA and Weight Loss
 Liver Failure, Acute Antibodies  >2 publications with ARSA and Liver Failure, Acute
 Testicular Diseases Antibodies  >2 publications with ARSA and Testicular Diseases

Organs/Tissues associated with anti-ARSA antibody

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >79 publications with ARSA and Vascular
 Blood Antibodies  >65 publications with ARSA and Blood
 Brain Antibodies  >39 publications with ARSA and Brain
 Heart Antibodies  >28 publications with ARSA and Heart
 Esophagus Antibodies  >20 publications with ARSA and Esophagus
 Nerve Antibodies  >16 publications with ARSA and Nerve
 Liver Antibodies  >14 publications with ARSA and Liver
 Muscle Antibodies  >11 publications with ARSA and Muscle
 Kidney Antibodies  >10 publications with ARSA and Kidney
 Trachea Antibodies  >9 publications with ARSA and Trachea
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000